Powder: -20°C for 3 years | In solvent: -80°C for 1 year
HET0016 is a potent and selective 20-HETE synthase inhibitor (IC50s: 17.7 nM, 12.1 nM, and 20.6 nM for recombinant CYP4A1-, CYP4A2- and CYP4A3-catalyzed 20-HETE synthesis). HET0016 also is a selective CYP450 inhibitor, which has been shown to inhibit angiogenesis and tumor growth.
产品描述 | HET0016 is a potent and selective 20-HETE synthase inhibitor (IC50s: 17.7 nM, 12.1 nM, and 20.6 nM for recombinant CYP4A1-, CYP4A2- and CYP4A3-catalyzed 20-HETE synthesis). HET0016 also is a selective CYP450 inhibitor, which has been shown to inhibit angiogenesis and tumor growth. |
靶点活性 | CYP4A1:17.7 nM , CYP4A2: 12.1 nM , CYP4A3: 20.6 nM |
体外活性 | HET0016 (100 μM; 24 hours, 48 hours) decreases migration and invasion of breast cancer metastatic cells .HET0016 is a selective, non-competitive and irreversible inhibitor of CYP4A . |
体内活性 | HET0016 decreases expression of pro-inflammatory and growth factors and granulocytic MDSCs population in lung microenvironment. HET0016 protects BBB dysfunction after I/R by regulating the expression of MMP-9 and tight junction proteins.HET0016 (10 mg/kg/day; i.v.; for 3 weeks) reduces tumor volume and lung metastasis in an immunocompetent breast cancer mouse model. HET0016 reduces the metalloproteinases' levels in the lungs via PI3K/AKT pathway in mice. |
分子量 | 206.28 |
分子式 | C12H18N2O |
CAS No. | 339068-25-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DCM: 12.5 mg/mL (60.60 mM), Sonication is recommended.
DMSO: 5 mg/mL (24.24 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DCM / DMSO | 1 mM | 4.8478 mL | 24.2389 mL | 48.4778 mL | 121.1945 mL |
5 mM | 0.9696 mL | 4.8478 mL | 9.6956 mL | 24.2389 mL | |
10 mM | 0.4848 mL | 2.4239 mL | 4.8478 mL | 12.1194 mL | |
20 mM | 0.2424 mL | 1.2119 mL | 2.4239 mL | 6.0597 mL | |
DCM | 50 mM | 0.097 mL | 0.4848 mL | 0.9696 mL | 2.4239 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
HET0016 339068-25-6 Others HET 0016 HET-0016 Inhibitor inhibitor inhibit