Powder: -20°C for 3 years | In solvent: -80°C for 1 year
4-Chlorophenylhydrazine hydrochloride (p-Chlorophenylhydrazine HCl) 用作药物中间体。
产品描述 | 4-Chlorophenylhydrazine hydrochloride (p-Chlorophenylhydrazine HCl) is used as a pharmaceutical intermediate. |
激酶实验 | ERK Inhibition Assay: Compounds are assayed for the inhibition of ERK2 by a spectophotometric coupled-enzyme assay. In this assay, a fixed concentration of activated ERK2 (10 nM) is incubated with various concentrations of the compounds in DMSO (2.5%) for 10 min. at 30 °C in 0.1 M HEPES buffer, pH = 7.5, containing 10 mM MgCl2, 2.5 mM phosphoenolpyruvate, 200 μM NADH, 150 μg/mL pyruvate kinase, 50 μg/mL lactate dehydrogenase and 200 μM erktide peptide. The reaction is initiated by the addition of 65 μM ATP. The rate of decrease of absorbance at 340 nM is monitored. The IC50 is evaluated from the data as a function of inhibitor concentr |
别名 | p-Chlorophenylhydrazine HCl, 对氯苯肼盐酸盐 |
分子量 | 179.05 |
分子式 | C6H8Cl2N2 |
CAS No. | 1073-70-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/mL (307.18 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 5.585 mL | 27.9252 mL | 55.8503 mL | 139.6258 mL |
5 mM | 1.117 mL | 5.585 mL | 11.1701 mL | 27.9252 mL | |
10 mM | 0.5585 mL | 2.7925 mL | 5.585 mL | 13.9626 mL | |
20 mM | 0.2793 mL | 1.3963 mL | 2.7925 mL | 6.9813 mL | |
50 mM | 0.1117 mL | 0.5585 mL | 1.117 mL | 2.7925 mL | |
100 mM | 0.0559 mL | 0.2793 mL | 0.5585 mL | 1.3963 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
4-Chlorophenylhydrazine hydrochloride 1073-70-7 Others 4Chlorophenylhydrazine hydrochloride 4-Chlorophenylhydrazine Hydrochloride p-Chlorophenylhydrazine HCl 对氯苯肼盐酸盐 4 Chlorophenylhydrazine hydrochloride Inhibitor inhibitor inhibit