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12-Oleanene-3,6-diol

产品编号 TN6586 CAS 41498-79-7

12-Oleanene-3,6-diol has antitumor activity, it can induce apoptosis and has inhibition effect on the proliferation in RKO cell line.

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12-Oleanene-3,6-diol, CAS 41498-79-7

规格	价格/CNY		货期	数量
5 mg	￥ 2,850		待询
1 mL * 10 mM (in DMSO)	￥ 2,950		待询

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天然产物信息

生物活性

化学信息

存储 & 溶解度

参考文献

植物来源	天然产物 > 卫矛科 > 南蛇藤属
结构类型	天然产物 > 其他

产品描述

12-Oleanene-3,6-diol has antitumor activity, it can induce apoptosis and has inhibition effect on the proliferation in RKO cell line.

体外活性

To investigate the effects of novel triterpene (12-Oleanene-3,6-diol ) from Celastrus hypoleucus on the proliferation and apoptosis of human colorectal cancer cell line RKO. METHODS AND RESULTS:The inhibitory effect of the novel triterpene on RKO cell proliferation was assayed by MTT dye reduction. The morphology of apoptotic cells was observed with AO/EB double fluorescence staining and HE staining, DNA fragment with electrophoresis on agarose gels, sub-diploid peak and cell cycle with flow cytometer (FCM). Novel triterpene (12-Oleanene-3,6-diol) from C. hypoleucus significantly inhibited proliferation of RKO cells in dose-dependent and time-dependent manner, the IC50 was (12.20 +/- 0.79) microg x mL(-1) at 48 h. Typical apoptotic changes were observed in RKO cells under the fluorescence microscope and the light microscope. DNA ladder was detected on agarose gels at concentrations from 10 microg x mL(-1) to 20 microg x mL(-1) at 48 h. With FCM methods, dose-dependent apoptosis-induced effect was observed in RKO cell line after treatment of triterpene for 48 h, and the apoptotic rates were increased from(2.93 +/- 0.84) % to (50.79 +/- 6.61) % at concentrations from 2.5 microg x mL(-1) to 20 microg x mL(-1). DNA histograms data from FCM analysis showed that the number of cells was obviously reduced during G0-G1 phase and G2-M phase, but not during S phase for RKO cell line after treatment with various concentrations of the triterpene for 48 hours. CONCLUSIONS:Novel triterpene (12-Oleanene-3,6-diol) from C. hypoleucus can induce apoptosis and has inhibition effect on the proliferation in RKO cell line.

分子量	442.72
分子式	C30H50O2
CAS No.	41498-79-7

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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参考文献

1. Antitumor effects of novel triterpene from Celastrus hypoleucus on human colorectal cancer cell line RKO in vitro. China journal of Chinese materia medica, 2006, 31(17):1450-1453.

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请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

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