Powder: -20°C for 3 years | In solvent: -80°C for 1 year
W146 TFA 是选择性 1-磷酸-鞘氨醇受体亚型 1 (S1PR1) 的拮抗剂,EC50值为 398 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 μg | ¥ 1,360 | 35日内发货 | ||
1 mg | ¥ 2,550 | 35日内发货 | ||
5 mg | ¥ 10,700 | 35日内发货 | ||
10 mg | ¥ 18,700 | 35日内发货 |
产品描述 | W146 TFA is a selective sphingosine-1-phosphate receptor 1 ( S1PR1 ) antagonist with an EC 50 value of 398 nM. |
体外活性 | W146 is a S1PR1 antagonist with a K i of ~70-80 nM [1]. W146 pretreatment significantly increases activated cleaved caspase-3 levels. The reduced apoptosis of EPCs induced by S1P is completely abolished after treatment with W146 [2]. Apoptosis Analysis [2] Cell Line: Endothelial progenitor cells (EPCs). Concentration: 10 μM. Incubation Time: 30 min before the addition of S1P. Result: Increases activated cleaved caspase-3 levels. |
体内活性 | W146 (5 mg/kg, ip, prior to AMD3100 administration) pre-treatment exhibits an approximately 8-fold increase of KSL-HSPC mobilization, measured by the CFU-G/M colony forming assays, compared to that in mice treated with AMD3100 alone [3] The W146-mediated augmentation of KSL-HSPC mobilization is specific, because pretreatment of mice with W140 is unable to produce any effect on AMD3100-stimulated KSL-HSPC mobilization. Injections of W146, W140, JTE013, or Cay10444 do not alter the basal WBC count in mice [3]. Animal Model: Mice (4-6-week-old) [3]. Dosage: 5 mg/kg. Administration: IP, 1 hour prior to AMD3100 (ADM) administration. Result: Significantly increased in KSL-HSPC mobilization compared to that in mice pretreated with dextran followed by AMD3100 administration. |
分子量 | 456.399 |
分子式 | C18H28F3N2O6P |
CAS No. | 909725-62-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
W146 TFA 909725-62-8 W-146 TFA Inhibitor inhibitor inhibit