Powder: -20°C for 3 years | In solvent: -80°C for 1 year
4′-Bromoflavone is a highly effective inducer of phase II detoxification enzymes and has been identified as a potent agent in cancer chemoprevention.
产品描述 | 4′-Bromoflavone is a highly effective inducer of phase II detoxification enzymes and has been identified as a potent agent in cancer chemoprevention. |
体外活性 | 4′-Bromoflavone significantly induces quinone reductase (QR) activity in cultured murine hepatoma 1c1c7 cells (concentration to double activity: 10 nM) and effectively induces the α- and mu-isoforms of glutathione S-transferase in cultured H4IIE rat hepatoma cells with no observed toxicity[1]. 4′-Bromoflavone is a potent inhibitor of cytochrome P4501A1-mediated ethoxyresorufin-O-deethylase activity, with an IC 50 of 0.86 μM. In HepG2 or MCF-7 cells, 4′-Bromoflavone significantly reduces the covalent binding of metabolically activated benzo[a]pyrene to cellular DNA[1]. |
体内活性 | In short-term dietary studies, 4′-Bromoflavone is also shown to increase QR activity and glutathione levels in rat liver, mammary gland, colon, stomach, and lung in a dose-dependent manner. In studies conducted with female Sprague Dawley rats ,4′-Bromoflavone significantly increases QR activity (phase II enzyme)[1]. In DMBA-treated female Sprague Dawley rats. Dietary administration of 4′-Bromoflavone (2000 and 4000 mg/kg of diet, from 1 week before to 1 week after DMBA) significantly inhibits the incidence and multiplicity of mammary tumors and greatly increases tumor latency[1]. |
分子量 | 301.14 |
分子式 | C15H9BrO2 |
CAS No. | 20525-20-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
4′-Bromoflavone 20525-20-6 4′Bromoflavone 4′ Bromoflavone Inhibitor inhibitor inhibit