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Nodinitib-1

Nodinitib-1

产品编号 T6591   CAS 799264-47-4
别名: ML130, CID-1088438

Nodinitib-1 (CID-1088438) 是 NOD1抑制剂(IC50:0.56 μM)。

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Nodinitib-1 Chemical Structure
Nodinitib-1, CAS 799264-47-4
规格 价格/CNY 货期 数量
1 mg ¥ 192 现货
5 mg ¥ 410 现货
10 mg ¥ 557 现货
25 mg ¥ 1,050 现货
50 mg ¥ 1,680 现货
100 mg ¥ 2,690 现货
200 mg ¥ 3,960 现货
1 mL * 10 mM (in DMSO) ¥ 433 现货
产品目录号及名称: Nodinitib-1 (T6591)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Nodinitib-1 (CID-1088438) is a potent and selective NOD1 inhibitor with an IC50 of 0.56 μM.
靶点活性 NOD1:0.56 μM
体外活性 ML130 has shown selective inhibition of NOD1-induced NF-κB activation in HEK293 cells with no cytotoxicity and is selected as a probe candidate molecule. ML130 is also confirmed in secondary assays by selectively inhibiting NOD1-dependent IL-8 secretion and also selectively inhibiting the NOD1-dependent pathway to NF-κB activation. [1] In another research, ML130 is proved to cause conformational changes of NOD1 in vitro and alter NOD1 subcellular targeting in cells, providing chemical probes for interrogating mechanisms regulating NOD1 activity and tools for exploring the roles of NOD1 in various infectious and inflammatory diseases. [2]
激酶实验 NOD1 Dose Response assay: Day 1 Procedure 1) Harvest HEK-293-T NFKB-Luc at 100% confluency at 100% confluency. 2) Add NOD assay media with Multidrop. 3) Spin down plates at 1000 rpm for 1 min in centrifuge. 4) Serial compound dilutions. 5) Add gamma-tri-DAP to cell suspension at 0.75 ug/mL. 6) Seed 13000 cells/well in 4 uL/well to full plate HEK-293-T NFKB-Luc to TC-treated plate. 7) Spin down plates 500 RPM for 5 min on centrifuge. 8) Lid Plates. Sandwich 4 plates between 2 lidded 384 plates filled with Water. 9) Wrap plates securely in single layer of Plastic Wrap. 10) Incubate overnight (14 hours) in 37 ℃ and 5% CO2 incubator. Day 2 Procedure 1) Add 3 ul/well of SteadyGlo solution with Multidrop. 2) Shake plates on a plate shaker for 20 min. 3) Spin plates 1000 RPM for 1 min using centrifuge. 4) Read luminescence.IC50 values are calculated using GraphPad Prism 5.0.The average Z' for the screen is 0.6, the signal to background is 11.1, signal to noise is 78.6 and signal to window is 6.0.
细胞实验 Hepatic toxicity of compounds is determined with Fa2N-4 immortalized human hepatocytes using the ATP-lite 1-step assay. Fa2N-4 cells are seeded at 50,000 cells/well, and incubated with a range of concentrations of the test compound (0.01 μM-50 μM) in MFE support medium for 24 h at 37 ℃, 5% CO2. At the end of the experiment D-luciferin and luciferase are added. The emitted luminescent signal produced as a result of the reaction with cellular ATP is captured on the Infinite M200 plate reader. The concentration of each compound that killed 50% of the cells (LC50) is calculated by non-linear regression analysis using a log (inhibitior) vs response equation with a variable slope, using the statistic software package Prism4.(Only for Reference)
别名 ML130, CID-1088438
分子量 287.34
分子式 C14H13N3O2S
CAS No. 799264-47-4

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

Ethanol: 2.9 mg/mL (10 mM)

DMSO: 28.7 mg/mL (100 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 3.4802 mL 17.401 mL 34.802 mL 87.0049 mL
5 mM 0.696 mL 3.4802 mL 6.9604 mL 17.401 mL
10 mM 0.348 mL 1.7401 mL 3.4802 mL 8.7005 mL
DMSO 20 mM 0.174 mL 0.87 mL 1.7401 mL 4.3502 mL
50 mM 0.0696 mL 0.348 mL 0.696 mL 1.7401 mL
100 mM 0.0348 mL 0.174 mL 0.348 mL 0.87 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Khan PM et al. ACS Med Chem Lett, 2011, 2(10), 780-785. 2. Correa RG et al. Chem Biol, 2011, 18(7), 825-832.

文献引用

1. Zhang Y, Li N, Yuan G, et al. Upregulation of NOD1 and NOD2 contribute to cancer progression through the positive regulation of tumorigenicity and metastasis in human squamous cervical cancer. BMC medicine. 2022, 20(1): 1-15.
NLRP3-IN-9 Muramyl dipeptide MCC950 Molsidomine Dapansutrile Emlenoflast sodium Lobeglitazone Sulfate MCC950 sodium

相关化合物库

该产品包含在如下化合物库中:
抗纤维化化合物库 抗乳腺癌化合物库 肿瘤免疫治疗小分子化合物库 抗衰老化合物库 细胞焦亡化合物库 经典已知活性库 血管生成库 抗卵巢癌化合物库 细胞凋亡化合物库 抗抑郁症化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Nodinitib-1 799264-47-4 Apoptosis Immunology/Inflammation NF-Κb TNF NOD-like Receptor (NLR) NOD ML130 Nodinitib1 Inhibitor CID 1088438 ML 130 CID-1088438 Nodinitib 1 CID1088438 ML-130 inhibit inhibitor

 

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