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Benzamil

产品编号 T5850 CAS 2898-76-2

别名: Benzamil (hydrochloride)

Benzamil 是 Amiloride 类似物，是 Na+/Ca2+交换体抑制剂，抑制 TRPP3 介导的 Ca2+激活电流，IC50为 1.1 μM。它也是一种非选择性上皮钠通道 (ENaC) 阻滞剂，能增强肌源性血管收缩。

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Benzamil, CAS 2898-76-2

规格	价格/CNY	货期
2 mg	￥ 277	现货
5 mg	￥ 397	现货
10 mg	￥ 587	现货
25 mg	￥ 997	现货
50 mg	￥ 1,690	现货
100 mg	￥ 2,535	现货
1 mL * 10 mM (in DMSO)	￥ 397	现货

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其他形式的 Benzamil:

Benzamil hydrochloride
询价

选择批次

纯度: 99.3%

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生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

产品描述	Benzamil is an epithelial sodium channel (ENaC) blocker that inhibits sodium transport from (IC50 = 4 nM) and binds to (Kd = 5 nM) bovine kidney cortex membrane vesicles.
体内活性	nd also examined the role of ASICs on modulation of neuropathic pain.?Neuropathic pain was induced by L4-5 spinal nerve ligation in male Sprague-Dawley rats weighing 100-120 g, and intrathecal catheterization was performed for drug administration.?The effects of amiloride and benzamil were measured by the paw-withdrawal threshold to a mechanical stimulus using the up and down method.?The expression of ASICs in the spinal cord dorsal horn was also analyzed by RT-PCR.?Intrathecal amiloride and benzamil significantly increased the paw withdrawal threshold in spinal nerve-ligated rats (87%±12% and 76%±14%, P=0.007 and 0.012 vs vehicle, respectively).?Spinal nerve ligation increased the expression of ASIC3 in the spinal cord dorsal horn (P=0.01), and this increase was inhibited by both amiloride and benzamil (P<0.001 in both).?Intrathecal amiloride and benzamil display antinociceptive effects in the rat spinal nerve ligation model[1].
动物实验	On the day of the experiment, rats were allocated into experimental and control groups for the tested drugs.?Control groups were performed using intrathecal DMSO (n=5) or methanol (n=5) according to the solvent used for the tested drugs.?All experiments were performed by investigators blinded to the treatment.?To assess the effects of both drugs, increasing doses of amiloride (1, 3, 10 μg in 10 μL, n=5-7) or benzamil (3, 10, 30 μg in 10 μL, n=5-7) were investigated.?The withdrawal threshold was measured prior to spinal nerve ligation and was regarded as pre-ligated threshold.?The withdrawal threshold was measured immediately prior to intrathecal drug delivery and was regarded as a post-ligated baseline threshold.?The withdrawal threshold was determined at 15, 30, 60, 90, 120, 150, and 180 min following intrathecal administration of the experimental drugs.For the purpose of examining the behavioral changes by amiloride and benzamil, the highest dose of each drug was administered intrathecally to 10 additional rats.?Motor function was assessed by the righting reflex and placing-stepping reflex[1].

别名	Benzamil (hydrochloride)
分子量	319.75
分子式	C13H14ClN7O
CAS No.	2898-76-2

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 3.2 mg/mL (10 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1274 mL	15.6372 mL	31.2744 mL	78.1861 mL
	5 mM	0.6255 mL	3.1274 mL	6.2549 mL	15.6372 mL
	10 mM	0.3127 mL	1.5637 mL	3.1274 mL	7.8186 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

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参考文献

1. Jeong S , Lee S H , Kim Y O , et al. Antinociceptive Effects of Amiloride and Benzamil in Neuropathic Pain Model Rats[J]. Journal of Korean Medical Science, 2013, 28(8). 2. Nishimura M , Ohtsuka K , Nanbu A , et al. Benzamil blockade of brain Na+ channels averts Na(+)-induced hypertension in rats.[J]. American Journal of Physiology, 1998, 274(2):635-44.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

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计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Benzamil 2898-76-2 Membrane transporter/Ion channel Na+/Ca2+ Exchanger Sodium Channel Inhibitor myogenic Benzylamiloride neuronal Na channels vasoconstriction Na+ channels channel podocytes inhibit Benzamil (hydrochloride) inhibitor

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Benzamil

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

相关产品

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剂量换算

体内实验配液计算器

技术支持

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