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Tubastatin A Hydrochloride

产品编号 T6161 CAS 1310693-92-5

别名: TSA HCl, Tubastatin A HCl

Tubastatin A Hydrochloride (TSA HCl) 是一种选择性的 HDAC6抑制剂，IC50值为 15 nM，对其选择性是 HDAC8 外的其他亚型的 1000 多倍。

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Tubastatin A Hydrochloride Chemical Structure

Tubastatin A Hydrochloride, CAS 1310693-92-5

规格	价格/CNY	货期
5 mg	￥ 339	现货
10 mg	￥ 551	现货
50 mg	￥ 677	现货
100 mg	￥ 1,192	现货
200 mg	￥ 1,995	现货
1 mL * 10 mM (in DMSO)	￥ 415	现货

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其他形式的 Tubastatin A Hydrochloride:

Tubastatin A
现货
Tubastatin A TFA
询价

选择批次

纯度: 99.25%

纯度: 97.55%

纯度: 97.24%

更多批次查询请联系客服

生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

产品描述	Tubastatin A Hydrochloride (TSA HCl) is an effective and specific HDAC6 inhibitor (IC50: 15 nM). It has selectivity (>1000-fold) against all other isozymes except HDAC8 (>57-fold).
靶点活性	HDAC6:15 nM
体外活性	Tubastatin A is substantially selective for all 11 HDAC isoforms and maintains over 1000-fold selectivity against all isoforms excluding HDAC8, where it has approximately 57-fold selectivity. In homocysteic acid (HCA) induced neurodegeneration assays, Tubastatin A displays dose-dependent protection against HCA-induced neuronal cell death starting at 5 μM with near complete protection at 10 μM. [1] Tubastatin A increases Foxp3+ T-regulatory cells (Tregs) suppression of T cell proliferation at 100 ng/mL in vitro. [2] Tubastatin A treatment in C2C12 cells would lead to myotube formation impairment when alpha-tubulin is hyperacetylated early in the myogenic process; however, myotube elongation occurs when alpha-tubulin is hyeperacetylated in myotubes. [3] A recent study indicates that Tubastatin A treatment increases cell elasticity as revealed by atomic force microscopy (AFM) tests without exerting drastic changes to the actin microfilament or microtubule networks in mouse ovarian cancer cell lines, MOSE-E and MOSE-L. [4]
体内活性	Daily treatment of 0.5 mg/kg Tubastatin A inhibits HDAC6 to promote Tregs suppressive activity in mouse models of inflammation and autoimmunity, which including multiple forms of experimental colitis and fully major histocompatibility complex (MHC)-incompatible cardiac allograft rejection. [2]
激酶实验	Enzyme Inhibition Assays: Enzyme inhibition assays are performed by the Reaction Biology Corporation, Malvern, PA, using the Reaction Biology HDAC Spectrum platform. (www.reactionbiology.com) The HDAC1, 2, 4, 5, 6, 7, 8, 9, 10, and 11 assays use isolated recombinant human protein; HDAC3/NcoR2 complex is used for the HDAC3 assay. Substrate for HDAC1, 2, 3, 6, 10, and 11 assays is a fluorogenic peptide from p53 residues 379-382 (RHKKAc); substrate for HDAC8 is fluorogenic diacyl peptide based on residues 379-382 of p53 (RHKAcKAc). Acetyl-Lys (trifluoroacetyl)-AMC substrate is used for HDAC4, 5, 7, and 9 assays. Tubastatin A is dissolved in DMSO and tested in 10-dose IC50 mode with 3-fold serial dilution starting at 30 μM. Control Compound Trichostatin A (TSA) is tested in a 10-dose IC50 with 3-fold serial dilution starting at 5 μM. IC50 values are extracted by curve-fitting the dose/response slopes.
细胞实验	Primary cortical neuron cultures are obtained from the cerebral cortex of fetal Sprague-Dawley rats (embryonic day 17) as described previously. All experiments are initiated 24 hours after plating. Under these conditions, the cells are not susceptible to glutamate-mediated excitotoxicity. For cytotoxicity studies, cells are rinsed with warm PBS and then placed in minimum essential medium (Invitrogen) containing 5.5 g/L glucose, 10% fetal calf serum, 2 mM L-glutamine, and 100 μM cystine. Oxidative stress is induced by the addition of the glutamate analogue homocysteate (HCA; 5 mM) to the media. HCA is diluted from 100-fold concentrated solutions that are adjusted to pH 7.5. In combination with HCA, neurons are treated with Tubastatin A at the indicated concentrations. Viability is assessed after 24 hours by MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method.(Only for Reference)

别名	TSA HCl, Tubastatin A HCl
分子量	371.86
分子式	C20H21N3O2·HCl
CAS No.	1310693-92-5

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 3.7 mg/mL (10 mM), Heating is recommended.

H2O: 7.43 mg/mL (20 mM）, Sonication and heating are recommended.

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO / H2O	1 mM	2.6892 mL	13.4459 mL	26.8918 mL	67.2296 mL
	5 mM	0.5378 mL	2.6892 mL	5.3784 mL	13.4459 mL
	10 mM	0.2689 mL	1.3446 mL	2.6892 mL	6.723 mL
H2O	20 mM	0.1345 mL	0.6723 mL	1.3446 mL	3.3615 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

溶液体积

质量

配液浓度

参考文献

1. Butler KV, et al, J Am Chem Soc, 2010, 132(31), 10842-10846. 2. de Zoeten EF, et al, Mol Cell Biol, 2011, 31(10), 2066-2078. 3. Di Fulvio S, et al, PloS One, 2011, 6(12):e28563. 4. Ketene AN, et al, Integr Biol (Camb), 2012, 4(5), 540-549.

文献引用

1. Liu S, Zhang H L, Li J, et al.Tubastatin A potently inhibits GPX4 activity to potentiate cancer radiotherapy through boosting ferroptosis.Redox Biology.2023: 102677.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Tubastatin A Hydrochloride 1310693-92-5 Apoptosis Autophagy Chromatin/Epigenetic DNA Damage/DNA Repair HDAC Tubastatin A Histone deacetylases Inhibitor inhibit TSA HCl Tubastatin A HCl inhibitor

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Tubastatin A Hydrochloride

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

文献引用

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords