Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PF-4708671 是一种可渗透细胞的 p70 核糖体 S6 激酶抑制剂,对 S6K1的Ki 为 20 nM,IC50为 160 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 298 | 现货 | ||
10 mg | ¥ 491 | 现货 | ||
25 mg | ¥ 961 | 现货 | ||
50 mg | ¥ 1,637 | 现货 | ||
100 mg | ¥ 2,767 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 539 | 现货 |
产品描述 | PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) .In cell-free assays, PF-4708671(PF4708671) is potent for S6K1(Ki50=20 nM, IC50=160 nM). |
靶点活性 | p70 S6K1:160 nM |
体内活性 | PF-4708671可抑制S6K1介导的S6蛋白对IGF-1(胰岛素样生长因子1)的磷酸化反应,还能够通过mTORC1(mTOR复合物1)诱导S6K1的T环和疏水基序的磷酸化。 |
激酶实验 | Affinity determination: Purified activated FAK kinase domain (amino acids 410–689) is reacted with 50 μM ATP, and 10 μg/well of a random peptide polymer of Glu and Tyr (molar ratio of 4:1), poly(Glu/Tyr) in kinase buffer (50 mM HEPES, pH 7.5, 125 mM NaCl, 48 mM MgCl2) for 15 min. Phosphorylation of poly(Glu/Tyr) is challenged with serially diluted compounds at 1/2-Log concentrations starting at a top concentration of 1 μM. Each concentration is run in triplicate. Phosphorylation of poly(Glu/Tyr) is detected with a general anti-phospho-tyrosine (PY20) antibody, followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody. The standard horseradish peroxidase substrate 3, 3', 5, 5'-tetramethylbenzidine is added, and Optical Density readings at 450 nm are obtained following the addition of stop solution (2 M H2SO4). The IC50 values are determined using the Hill slope model. |
别名 | PF4708671 |
分子量 | 390.41 |
分子式 | C19H21F3N6 |
CAS No. | 1255517-76-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 19.5 mg/mL (50 mM)
Ethanol: 19.5 mg/mL (50 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 2.5614 mL | 12.807 mL | 25.6141 mL | 64.0352 mL |
5 mM | 0.5123 mL | 2.5614 mL | 5.1228 mL | 12.807 mL | |
10 mM | 0.2561 mL | 1.2807 mL | 2.5614 mL | 6.4035 mL | |
20 mM | 0.1281 mL | 0.6404 mL | 1.2807 mL | 3.2018 mL | |
50 mM | 0.0512 mL | 0.2561 mL | 0.5123 mL | 1.2807 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
PF-4708671 1255517-76-0 Autophagy MAPK PI3K/Akt/mTOR signaling S6 Kinase inhibit S6K Inhibitor PF4708671 Ribosomal S6 Kinase (RSK) PF 4708671 inhibitor