Powder: -20°C for 3 years | In solvent: -80°C for 1 year
IOWH-032 (IOWH032) 是一种合成 CFTR 抑制剂,可用于研究治疗霍乱、腹泻和分泌性腹泻,在T84和CHO-CFTR 细胞实验中IC50为1.01 uM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 262 | 现货 | ||
10 mg | ¥ 492 | 现货 | ||
25 mg | ¥ 852 | 现货 | ||
50 mg | ¥ 1,347 | 现货 | ||
100 mg | ¥ 1,980 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 321 | 现货 |
产品描述 | IOWH-032 (IOWH032) , a synthetic CFTR inhibitor, has been investigated for the treatment of cholera, diarrhea, and secretory diarrhea. |
靶点活性 | CFTR:1.01 μM |
体外活性 | 在老鼠封闭系统模型中,IOWH032能够抑制霍乱毒素诱发的分泌物.切除盲肠的大鼠模型中, IOWH032(5 mg/kg)抑制排泄物排出. |
体内活性 | 在T84-CFTR细胞(IC50=6.87 μM)中,IOWH032能够抑制CFTR。 |
激酶实验 | Enzyme assays are performed using a homogeneous time-resolved fluorescence assay with recombinant epitope tagged kinase domains (JAK1, 837-1142; JAK2, 828-1132; JAK3, 718-1124; Tyk2, 873-1187) or full-length enzyme (cMET and Chk2) and peptide substrate. Each enzyme reaction is performed with or without test compound (11-point dilution), JAK, cMET, or Chk2 enzyme, 500 nM (100 nM for Chk2) peptide, ATP (at the Km specific for each kinase or 1 mM), and 2.0% DMSO in assay buffer. The calculated IC50 value is the compound concentration required for inhibition of 50% of the fluorescent signal. Additional kinase assays are performed at Cerep using standard conditions at 200 nM. Enzymes tested included: Abl, Akt1, AurA, AurB, CDC2, CDK2, CDK4, CHK2, c-kit, EGFR, EphB4, ERK1, ERK2, FLT-1, HER2, IGF1R, IKKα, IKKβ, JNK1, Lck, MEK1, p38α, p70S6K, PKA, PKCα, Src, and ZAP70[1]. |
别名 | IOWH032, IOWH 032 |
分子量 | 545.18 |
分子式 | C22H15Br2N3O4 |
CAS No. | 1191252-49-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 93 mg/mL (170.6 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.8343 mL | 9.1713 mL | 18.3426 mL | 45.8564 mL |
5 mM | 0.3669 mL | 1.8343 mL | 3.6685 mL | 9.1713 mL | |
10 mM | 0.1834 mL | 0.9171 mL | 1.8343 mL | 4.5856 mL | |
20 mM | 0.0917 mL | 0.4586 mL | 0.9171 mL | 2.2928 mL | |
50 mM | 0.0367 mL | 0.1834 mL | 0.3669 mL | 0.9171 mL | |
100 mM | 0.0183 mL | 0.0917 mL | 0.1834 mL | 0.4586 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
IOWH-032 1191252-49-9 Autophagy Membrane transporter/Ion channel CFTR inhibit SARS-CoV Inhibitor Cystic fibrosis transmembrane conductance regulator SARS coronavirus anti-diarrheal CFBE41o- WT cells IOWH032 ACE-2 IOWH 032 inhibitor