keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SN50 is a cell permeable NF-κB translocation inhibitor. 在相同实验条件下,摩尔浓度相同的化合物盐形式与游离态具有相同的生物活性,但盐形式 SN50 acetate (213546-53-3 free base) 的水溶性和稳定性通常比游离态更好。
产品描述 | SN50 is a cell permeable NF-κB translocation inhibitor. |
体外活性 | Compared with vehicle-treated groups,pretreatment with SN50 results in a significant reduction in amount of PI-positive cells at 12, 24, and 48 h time-point post TBI [1]. Local SN50 inhibits the activation of nuclear factor-κB in local cells and reduces the occurrence of epithelial defects/ulcers during corneal healing. Myofibroblast generation, macrophage invasion, activity of matrix metalloproteinases, basement membrane destruction, and expression of cytokines are all decreased in treated corneas compared with controls[2]. Treating the human gastric cancer cells SGC7901 with SN50 could significantly enhance the effects of LY294002 on inducing cell death after 24 h[3]. SN50 can inhibit translocation of NF-kB and production of inflammatory cytokines that are implicated in lipopolysaccharide (LPS)-induced lung injury[4]. |
体内活性 | SN50 accelerates the recovery of motor functional outcome from 1st to 4th day. Animals subjected to SN50 pretreatment demonstrate a significant decrease in the visuospatial learning latencies relative to the control group at 7 and 8 days post-TBI. Pretreatment with SN50 results in a significant reduction of NF-κB p65 protein levels from 6 to 48 h post-TBI and TNF-a protein levels from 12 to 48 h post-TBI[1]. |
分子量 | 2781.5 |
分子式 | C129H230N36O29S |
CAS No. | 213546-53-3 |
keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 50 mg/mL (17.98 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 0.3595 mL | 1.7976 mL | 3.5952 mL | 8.988 mL |
5 mM | 0.0719 mL | 0.3595 mL | 0.719 mL | 1.7976 mL | |
10 mM | 0.036 mL | 0.1798 mL | 0.3595 mL | 0.8988 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SN50 213546-53-3 NF-Κb NF-κB SN-50 SN 50 Inhibitor inhibitor inhibit