Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Probenecid (Benemid) 是一种苯甲酸衍生物,具有抗高尿酸特性。它也是一种选择性瞬时受体电位香草酸受体通道 2 激动剂,还可抑制pannexin 1通道。
产品描述 | Probenecid (Benemid) is a benzoic acid derivative with antihyperuricemic property. |
体外活性 | Probenecid是肾脏,肝脏和眼睛中单胺转运的竞争性抑制剂.Probenecid能够降低肾脏对抗生素的清除,这能够增强了血清中抗生素的水平.Probenecid作为OTA竞争性抑制剂,这阻止了OAT介导的尿酸从尿液中再摄取进入血清,增强肾脏中尿酸的排泄. |
体内活性 | 在1321N1星形细胞瘤中,Probenecid(2.5 mM) 能够阻断Fura-2从流出,并且不改变基底钙离子浓度或毒蕈碱钙响应。 |
细胞实验 | Probenecid is dissolved at 500 mM in 1 N NaOH and titrated to pH 7.0[1]. HEK-293T cells are transfected with hTAS2R expression constructs using Lipofectamine 2000 in poly-lysine coated, black 384-well plates with clear bottoms and incubated for 22 hours at 37°C. Growth media is removed and cells are washed twice with HBSS containing 20 mM HEPES, then loaded with a calcium indicator dye in HBSS containing 20 mM HEPES (Calcium 4 Assay kit) with or without 1 mM Probenecid. Cells are incubated at 37°C for 1 hour in the presence of both dye and Probenecid, then moved to a Flexstation II-384 set for 32°C. After a 15-minute temperature equilibration (without washout), indicated compounds are injected (at t=~25 seconds) and fluorescence is measured for 100 to 180 seconds, reading every 3 seconds. Data sets are analyzed and represented as % over baseline signal using Prism 5.0 software. For Schild plots, replicates of raw calcium flux values are expressed as % over baseline signal. The mean value at 36 seconds (corresponding to the maximum flux signal) for each concentration of TAS2R ligand in the presence of the indicated concentration of Probenecid is plotted against the log of ligand concentration. Data points are fit using non-linear regression in GraphPad Prism[1]. |
别名 | Benemid, 丙磺舒 |
分子量 | 285.36 |
分子式 | C13H19NO4S |
CAS No. | 57-66-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 53 mg/mL (185.7 mM)
Ethanol: 21 mg/mL (73.6 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.5043 mL | 17.5217 mL | 35.0435 mL | 87.6086 mL |
5 mM | 0.7009 mL | 3.5043 mL | 7.0087 mL | 17.5217 mL | |
10 mM | 0.3504 mL | 1.7522 mL | 3.5043 mL | 8.7609 mL | |
20 mM | 0.1752 mL | 0.8761 mL | 1.7522 mL | 4.3804 mL | |
50 mM | 0.0701 mL | 0.3504 mL | 0.7009 mL | 1.7522 mL | |
DMSO | 100 mM | 0.035 mL | 0.1752 mL | 0.3504 mL | 0.8761 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Probenecid 57-66-9 Membrane transporter/Ion channel Microbiology/Virology Proteases/Proteasome HIV Protease Antibacterial TRP/TRPV Channel Benemid Bacterial inhibit 丙磺舒 Inhibitor Human immunodeficiency virus TRP Channel HIV Transient receptor potential channels inhibitor