Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Kobe0065 是一种新型 Ras-Raf 相互作用抑制剂,可完全抑制 H-Ras·GTP 与 c-Raf-1 RBD 的结合,Ki 值为 46±13 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 152 | 现货 | ||
5 mg | ¥ 337 | 现货 | ||
10 mg | ¥ 543 | 现货 | ||
25 mg | ¥ 967 | 现货 | ||
50 mg | ¥ 1,560 | 现货 | ||
100 mg | ¥ 2,480 | 现货 | ||
200 mg | ¥ 3,930 | 现货 | ||
500 mg | ¥ 6,280 | 现货 |
产品描述 | Kobe0065 is a novel and effective small-molecule compound inhibiting Ras–Raf interaction by SBDD; shows potent activity to competitively inhibit the binding of H-Ras·GTP to c-Raf-1 RBD with a Ki value of 46 ± 13 μM. |
靶点活性 | C-Raf1 RBD:46 ± 13 μM(ki) |
体外活性 | Kobe0065-family compounds bind to Ras?GTP and exhibit antiproliferative activity toward cancer cells carrying the activated ras oncogenes. The compounds efficiently inhibit the interaction of Ras?GTP with their multiple effectors including Raf, PI3K, and RalGDS and a regulator/effector Sos and show rather broad binding specificity toward various Ras family small GTPases, which may account for their higher potency at the cellular level compared with that of the in vitro binding inhibition[1]. |
体内活性 | Kobe0065 has antitumor activity. The compound-treated tumors show a prominent increase of apoptotic cells without any significant body weight loss[1]. |
激酶实验 | Determination of lipase activity: For determination of lipase activity, lysates are incubated with a substrate containing radiolabeled [9,10-3H(N)]-triolein. Subsequently, FA are extracted and quantitated by liquid scintilation counting. Data are presented as mean S.D. of triplicate determinations and representative for at least three independent experiments. |
细胞实验 | NIH 3T3 cells are transfected with pEF-BOS-HA-H-Ras(G12V) or an empty vector and treated with the 2 and 20 μM compound or the vehicle (DMSO) in the presence of 2% FBS for 1 h. Cell lysate is subjected to detection of c-Raf-1 coimmunoprecipitated with an anti-H-Ras antibody and total c-Raf-1 by Western blotting with an anti-c-Raf-1 antibody. Immunoprecipitated H-Ras(G12V) are detected by an anti-HA antibody.(Only for Reference) |
分子量 | 449.79 |
分子式 | C15H11ClF3N5O4S |
CAS No. | 436133-68-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 4.5 mg/mL (10 mM)), Heating is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol | 1 mM | 2.2233 mL | 11.1163 mL | 22.2326 mL | 55.5815 mL |
5 mM | 0.4447 mL | 2.2233 mL | 4.4465 mL | 11.1163 mL | |
10 mM | 0.2223 mL | 1.1116 mL | 2.2233 mL | 5.5581 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Kobe0065 436133-68-5 Apoptosis GPCR/G Protein MAPK Raf Ras inhibit Inhibitor Kobe-0065 Kobe 0065 inhibitor