Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ID-8 是 DYRK 抑制剂,长时间培养能够维持胚胎干细胞的自我更新。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 153 | 现货 | ||
5 mg | ¥ 328 | 现货 | ||
10 mg | ¥ 538 | 现货 | ||
25 mg | ¥ 968 | 现货 | ||
50 mg | ¥ 1,530 | 现货 | ||
100 mg | ¥ 2,450 | 现货 | ||
200 mg | ¥ 3,620 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 363 | 现货 |
产品描述 | ID-8, a DYRK inhibitor, sustains embryonic stem cell self-renewal in long-term culture. |
体内活性 | ID-8通过Sox2-Oct3 / 4激活维持Nanog基因表达,能够可逆地维持ESC细胞的长期培养。ID-8在Wnt存在的情况下,调节Wnt /β-连环蛋白信号,增加CBP /β-连环蛋白,来维持hESC的未分化状态,并维持细胞的增殖和存活。 |
激酶实验 | In Vitro Enzymatic Assay for Histone Deacetylases: In vitro activities of the 11 recombinant human zinc-dependent HDAC enzymes are detected by ?uorigenic release of 7-amino-4-methylcoumarin from substrate upon deacetylase enzymatic activity. A series of dilutions of the unique HDAC6 compound, tubacin, and SAHA are prepared with 10% DMSO in HDAC assay buffer, and 5 μL of the dilution was added to a 50-μL reaction so that the ?nal concentration of DMSO is 1% in all of the reactions. The enzymatic reactions are conducted in duplicate at 37 °C for 30 min in a 50-μL mixture containing HDAC assay buffer, 5 μg BSA, an HDAC substrate, an HDAC enzyme, and a test compound. After enzymatic reactions, 50 μL of 2× HDAC developer is added to each well, and the plate is incubated at room temperature for an additional 15 min. Fluorescence intensity is measured at an excitation of 360 nm and an emission of 460 nm using a Synergy microplate reader. Negative (no enzyme, no inhibitor, a drug with no HDAC inhibition activity) and positive controls (known HDAC inhibitor SAHA) are included in the assays. IC50 is determined at the drug concentration that results in 50% reduction of HDAC activity compared with the control. |
细胞实验 | Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture. (Only for Reference) |
别名 | ID8 |
分子量 | 298.29 |
分子式 | C16H14N2O4 |
CAS No. | 147591-46-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 29.8 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.3524 mL | 16.7622 mL | 33.5244 mL | 83.8111 mL |
5 mM | 0.6705 mL | 3.3524 mL | 6.7049 mL | 16.7622 mL | |
10 mM | 0.3352 mL | 1.6762 mL | 3.3524 mL | 8.3811 mL | |
20 mM | 0.1676 mL | 0.8381 mL | 1.6762 mL | 4.1906 mL | |
50 mM | 0.067 mL | 0.3352 mL | 0.6705 mL | 1.6762 mL | |
100 mM | 0.0335 mL | 0.1676 mL | 0.3352 mL | 0.8381 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ID-8 147591-46-6 Cell Cycle/Checkpoint Tyrosine Kinase/Adaptors DYRK cell embryonic dual-specificity ESC Inhibitor inhibit Dual specificity tyrosine phosphorylation regulated kinase stem regulated phosphorylation kinase tyrosine ID 8 ID8 Dual specificity tyrosine regulated kinase inhibitor