Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sulfosuccinimidyl oleate (Sulfo-N-succinimidyl oleate) (Sulfo-N-succinimidyl oleate) 是一种长链脂肪酸,可抑制脂肪酸运输到细胞。Sulfosuccinimidyl oleate 是一种有效且不可逆的线粒体呼吸链抑制剂。Sulfosuccinimidyl oleate 与小胶质细胞表面上的CD36受体结合,具有抗炎效果。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 7,000 | 5日内发货 | ||
50 mg | ¥ 13,800 | 5日内发货 | ||
100 mg | ¥ 17,500 | 5日内发货 |
Sulfosuccinimidyl oleate 的其他形式现货产品:
产品描述 | Sulfosuccinimidyl oleate (Sulfo-N-succinimidyl oleate) (Sulfo-N-succinimidyl oleate) is a long chain fatty acid that inhibits fatty acid transport into cells. Sulfosuccinimidyl oleate is a potent and irreversible inhibitor of mitochondrial respiratory chain. Sulfosuccinimidyl oleate binds the CD36 receptor on the surface of microglia. Sulfosuccinimidyl oleate is neuroprotective and alleviates stroke-induced neuroinflammation [1] [2]. |
体外活性 | Sulfosuccinimidyl oleate (20 μM and 50 μM, 24 hours) alone does not change the cellular viability. Exposure to 100 ng/ml LPS+5 ng/mL IFNγ modestly, yet significantly decreases the viability of the BV2 cells. Co-treatment with Sulfosuccinimidyl oleate prevents the LPS+IFNγ-induced reduction in the cell viability [1]. Sulfosuccinimidyl oleate (50 μM, 24 hours) co-treatment significantly reduces the LPS+IFNγ-induced expression of NOS2 and COX-2 in BV2 cells. Western blot analysis reveals a significant LPS/IFNγ-induced upregulation in the phosphorylated form of the p38, which is prevented by co-treatment with Sulfosuccinimidyl oleate (50 μM, 24 hours) [1]. Cell Viability Assay [1] Cell Line: BV2 cells Concentration: 20 μM and 50 μM Incubation Time: 24 hours Result: Did not alter the viability of BV2 cells alone. Exposure of BV2 cells to 100 ng/mL LPS and 5 ng/mL IFNγ significantly reduced the viability of BV2 cells while simultaneous treatment with Sulfosuccinimidyl oleate prevented it. Western Blot Analysis [1] Cell Line: BV2 cells Concentration: 50 μM Incubation Time: 24 hours Result: Drastically increased the levels of NOS2, COX-2, and P-p38/T-p38. |
体内活性 | Sulfosuccinimidyl oleate (50 mg/kg; administered once by single oral gavage) significantly reduces the cortical ischemic infarct size compared to vehicle-treated controls in male BALB/cABom mice with pMCAo model. Moreover, Sulfosuccinimidyl oleate at 50 mg/kg is suitable to see a beneficial effect after stroke [1]. Animal Model: 4-month-old male BALB/cABom mice with pMCAo model [1] Dosage: 50 mg/kg Administration: Administered once by single oral gavage Result: Reduced brain damage following ischemia. Attenuated infarct size. |
别名 | Sulfo-N-succinimidyl oleate |
分子量 | 459.6 |
分子式 | C22H37NO7S |
CAS No. | 135661-44-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Sulfosuccinimidyl oleate 135661-44-8 Autophagy Mitophagy inhibit Mitochondrial Autophagy Inhibitor Sulfo-N-succinimidyl oleate inhibitor