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NSC 185058

NSC 185058

产品编号 T5211   CAS 39122-38-8
别名: NSC185058

NSC 185058 是一种半胱氨酸蛋白酶 ATG4B 抑制剂,可抑制自噬

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NSC 185058 Chemical Structure
NSC 185058, CAS 39122-38-8
规格 价格/CNY 货期 数量
1 mg ¥ 692 现货
5 mg ¥ 1,760 现货
10 mg ¥ 2,970 现货
25 mg ¥ 4,950 现货
50 mg ¥ 6,870 现货
100 mg ¥ 9,270 现货
产品目录号及名称: NSC 185058 (T5211)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 NSC 185058 is an inhibitor of ATG4B, a major cysteine protease. It also can attenuate the autophagic activity.
体外活性 NSC185058 effectively suppressed starvation-induced autophagy in both cell types. GFP-LC3B labeled AVs were absent in starved MDA-MB468 cells when treated with NSC185058. The inhibitory effects of both NSC185058 on starvation-induced protein turnover were comparable to 3MA.
体内活性 Mice were injected at 2 24 h intervals with vehicle or NSC185058, autophagy induced by 20 h fasting. Fasting resulted in an increase of GFP-LC3B AVs compared with ad libitum fed mice. NSC185058 significantly decreased the appearance of GFP-LC3B AVs compared with fasted mice.
细胞实验 Saos-2 transiently expressing FYVE-RFP was incubated in DMEM medium supplemented with amino acids and serum (Fed) or Krebs-Heinseleit medium lacking amino acids and serum (Starved). The starved cells were incubated in the presence and absence of the NSC185058 or NSC377071 for 4 h at which time the cells were examined by fluorescence microscopy. The numbers of red "dots" per cell was quantified using ImageJ software. Taking into account basal responses, those cells with 3 or more puncta were defined as PtdIns3K active.
动物实验 Immunodeficient nu/nu nude female mice were injected subcutaneously with 6 × 10^6 Saos-2 (GFP-LC3B), shCon-Saos-2(GFP-LC3B) or shATG4B-Saos-2(GFP-LC3B) cells. Palpable Saos-2 (GFP-LC3B) tumors developed in 7 to 10 d, at which time, the mice were divided into 2 groups and injected IP on Monday, Wednesday, and Friday with either peanut oil vehicle or NSC185058 (100 mg/kg body weight) dissolved in peanut oil. Over time, the tumor dimensions were measured using calipers and volumes calculated using the formula: (W2 × L)/2.
别名 NSC185058
分子量 215.27
分子式 C11H9N3S
CAS No. 39122-38-8

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 30 mg/mL (139.36 mM)

Ethanol: 2.5 mg/mL

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.6453 mL 23.2266 mL 46.4533 mL 116.1332 mL
5 mM 0.9291 mL 4.6453 mL 9.2907 mL 23.2266 mL
10 mM 0.4645 mL 2.3227 mL 4.6453 mL 11.6133 mL
20 mM 0.2323 mL 1.1613 mL 2.3227 mL 5.8067 mL
50 mM 0.0929 mL 0.4645 mL 0.9291 mL 2.3227 mL
100 mM 0.0465 mL 0.2323 mL 0.4645 mL 1.1613 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Akin D, et al. A novel ATG4B antagonist inhibits autophagy and has a negative impact on osteosarcoma tumors. Autophagy. 2014;10(11):2021-35.
TPEN Torin 2 Nidufexor Salbutamol hemisulfate Thalidomide 3-Bromopyruvic acid VISTA-IN-2 ALLO-1

相关化合物库

该产品包含在如下化合物库中:
已知活性化合物库 自噬库 抑制剂库 经典已知活性库 蛋白酶抑制剂库 NO PAINS 化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

NSC 185058 39122-38-8 Autophagy Proteases/Proteasome Cysteine Protease Inhibitor NSC185058 Atg4 Autophagy-related 4 NSC-185058 inhibit inhibitor

 

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