Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NSC 185058 是一种半胱氨酸蛋白酶 ATG4B 抑制剂,可抑制自噬。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 692 | 现货 | ||
5 mg | ¥ 1,760 | 现货 | ||
10 mg | ¥ 2,970 | 现货 | ||
25 mg | ¥ 4,950 | 现货 | ||
50 mg | ¥ 6,870 | 现货 | ||
100 mg | ¥ 9,270 | 现货 |
产品描述 | NSC 185058 is an inhibitor of ATG4B, a major cysteine protease. It also can attenuate the autophagic activity. |
体外活性 | NSC185058 effectively suppressed starvation-induced autophagy in both cell types. GFP-LC3B labeled AVs were absent in starved MDA-MB468 cells when treated with NSC185058. The inhibitory effects of both NSC185058 on starvation-induced protein turnover were comparable to 3MA. |
体内活性 | Mice were injected at 2 24 h intervals with vehicle or NSC185058, autophagy induced by 20 h fasting. Fasting resulted in an increase of GFP-LC3B AVs compared with ad libitum fed mice. NSC185058 significantly decreased the appearance of GFP-LC3B AVs compared with fasted mice. |
细胞实验 | Saos-2 transiently expressing FYVE-RFP was incubated in DMEM medium supplemented with amino acids and serum (Fed) or Krebs-Heinseleit medium lacking amino acids and serum (Starved). The starved cells were incubated in the presence and absence of the NSC185058 or NSC377071 for 4 h at which time the cells were examined by fluorescence microscopy. The numbers of red "dots" per cell was quantified using ImageJ software. Taking into account basal responses, those cells with 3 or more puncta were defined as PtdIns3K active. |
动物实验 | Immunodeficient nu/nu nude female mice were injected subcutaneously with 6 × 10^6 Saos-2 (GFP-LC3B), shCon-Saos-2(GFP-LC3B) or shATG4B-Saos-2(GFP-LC3B) cells. Palpable Saos-2 (GFP-LC3B) tumors developed in 7 to 10 d, at which time, the mice were divided into 2 groups and injected IP on Monday, Wednesday, and Friday with either peanut oil vehicle or NSC185058 (100 mg/kg body weight) dissolved in peanut oil. Over time, the tumor dimensions were measured using calipers and volumes calculated using the formula: (W2 × L)/2. |
别名 | NSC185058 |
分子量 | 215.27 |
分子式 | C11H9N3S |
CAS No. | 39122-38-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 30 mg/mL (139.36 mM)
Ethanol: 2.5 mg/mL
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 4.6453 mL | 23.2266 mL | 46.4533 mL | 116.1332 mL |
5 mM | 0.9291 mL | 4.6453 mL | 9.2907 mL | 23.2266 mL | |
10 mM | 0.4645 mL | 2.3227 mL | 4.6453 mL | 11.6133 mL | |
20 mM | 0.2323 mL | 1.1613 mL | 2.3227 mL | 5.8067 mL | |
50 mM | 0.0929 mL | 0.4645 mL | 0.9291 mL | 2.3227 mL | |
100 mM | 0.0465 mL | 0.2323 mL | 0.4645 mL | 1.1613 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NSC 185058 39122-38-8 Autophagy Proteases/Proteasome Cysteine Protease Inhibitor NSC185058 Atg4 Autophagy-related 4 NSC-185058 inhibit inhibitor