Powder: -20°C for 3 years | In solvent: -80°C for 1 year
7,8-Dihydroxyflavone (7,8-DHF) 是一种选择性有效的TrkB 激动剂,其模拟脑源性神经营养因子的生理作用。它有潜力研究各种神经疾病。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 210 | 现货 | ||
25 mg | ¥ 375 | 现货 | ||
50 mg | ¥ 671 | 现货 | ||
100 mg | ¥ 981 | 现货 | ||
500 mg | ¥ 2,480 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 248 | 现货 |
产品描述 | 7,8-Dihydroxyflavone (7,8-DHF) is a naturally-occurring flavone and exist in Tridax procumbens, Godmania aesculifolia, and primula tree leaves. |
靶点活性 | TrkB receptor:320 nM(Kd) |
体外活性 | 7,8-DHF is one of the positive compounds that specifically activate TrkB, but not TrkA or TrkC, at a concentration of 250 nM. In addition to cortical and hippocampal neurons, 7,8-DHF also protects other cell types including the RGC (retinal ganglion cells) and PC12 cells from excitotoxic and oxidative stress-induced apoptosis and cell death. Thus, it has neuroprotective properties[1]. |
体内活性 | 7,8-Dihydroxyflavone is a bioavailable chemical that can pass through the BBB to provoke TrkB and its downstream PI3K/Akt and MAPK activation in mouse brain upon intraperitoneal or oral administration. 7,8-DHF promotes the survival and reduces apoptosis in cortical neurons of traumatic brain injury as administration of 7,8-DHF at 3 h post-injury reduces brain tissue damage via the PI3K/Akt pathway. Its treatment does not induce any apparent toxicity in mice and is not toxic to the mice during the chronic treatment. 7,8-DHF displays robust therapeutic efficacy toward Alzheimer's disease and inhibits obesity through activating muscular TrkB[1]. |
细胞实验 | PC12 cells are seeded in 96-well plates at 104/well. After pretreatment with 7,8-DHF (1-25 μM) for 1 h, the cells are exposed to 6-OHDA (100 μM) for subsequent 24 h. The PI3k inhibitor LY294002 or MEK inhibitor PD98059 is added 30 min before 7,8-DHF treatment. At the end of the experiment, PC12 cells are incubated with 20 μl of MTT solution (5 mg/ml in PBS) for 4 h at 37 ?C. The dark blue formazan product due to the reduction of MTT is dissolved in 150 μl of DMSO, and the absorbance at 570 nm is recorded with a microplate reader. The viability is expressed as the percentage of the untreated control cells. (Only for Reference) |
别名 | 二羟基黄酮水合物, 7,8-DHF |
分子量 | 254.24 |
分子式 | C15H10O4 |
CAS No. | 38183-03-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 47 mg/mL (184.9 mM)
Ethanol: 1 mg/mL (3.93 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.9333 mL | 19.6665 mL | 39.3329 mL | 98.3323 mL |
DMSO | 5 mM | 0.7867 mL | 3.9333 mL | 7.8666 mL | 19.6665 mL |
10 mM | 0.3933 mL | 1.9666 mL | 3.9333 mL | 9.8332 mL | |
20 mM | 0.1967 mL | 0.9833 mL | 1.9666 mL | 4.9166 mL | |
50 mM | 0.0787 mL | 0.3933 mL | 0.7867 mL | 1.9666 mL | |
100 mM | 0.0393 mL | 0.1967 mL | 0.3933 mL | 0.9833 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
7,8-Dihydroxyflavone 38183-03-8 Apoptosis Tyrosine Kinase/Adaptors Trk receptor Trk Receptor 二羟基黄酮水合物 Inhibitor Tropomyosin related kinase receptor 7,8-DHF inhibit 7,8Dihydroxyflavone 7,8 Dihydroxyflavone inhibitor