Powder: -20°C for 3 years | In solvent: -80°C for 1 year
m-3M3FBS 是一种有效的磷脂酶C 激活剂,刺激人中性粒细胞产生超氧物,上调细胞内钙离子浓度,刺激多种细胞产生磷酸肌醇。它诱导单核细胞白血病细胞凋亡。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 233 | 现货 | ||
5 mg | ¥ 398 | 现货 | ||
10 mg | ¥ 663 | 现货 | ||
25 mg | ¥ 1,330 | 现货 | ||
50 mg | ¥ 2,490 | 现货 | ||
100 mg | ¥ 3,730 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 455 | 现货 |
产品描述 | m-3M3FBS is a phospholipase C (PLC) activator. |
体外活性 | m-3M3FBS stimulated a transient intracellular calcium concentration ([Ca(2+)](i)) increase in neutrophils.?Moreover, m-3M3FBS stimulated the formation of inositol phosphates in U937 cells, indicating that it stimulates PLC activity.?The compound showed no cell-type specificity in terms of [Ca(2+)](i) increase in the various cell lines including leukocytes, fibroblasts, and neuronal cells,?also ruled out the possible involvement of heterotrimeric G proteins in m-3M3FBS-stimulated signaling by confirming the following: 1) pertussis toxin does not inhibit m-3M3FBS-induced [Ca(2+)](i) increase;?2) m-3M3FBS does not stimulate cyclic AMP generation;?and 3) the inhibition of G(q) by the regulator of G protein-signaling 2 does not affect the m-3M3FBS-induced [Ca(2+)](i) increase.?m-3M3FBS stimulated PLC activity in vitro.?The purified isoforms of PLC that were tested (i.e., beta2, beta3, gamma1, gamma2, and delta1) were activated by m-3M3FBS and showed no isoform specificity[1]. |
别名 | 2,4,6-三甲基-N-[3-(三氟甲基)苯基]苯磺酰胺 |
分子量 | 343.36 |
分子式 | C16H16F3NO2S |
CAS No. | 200933-14-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 4.4 mg/mL (12.81 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.9124 mL | 14.562 mL | 29.124 mL | 72.8099 mL |
5 mM | 0.5825 mL | 2.9124 mL | 5.8248 mL | 14.562 mL | |
10 mM | 0.2912 mL | 1.4562 mL | 2.9124 mL | 7.281 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
m-3M3FBS 200933-14-8 Apoptosis Metabolism Phospholipase calcium superoxide cells THP-1 2,4,6-三甲基-N-[3-(三氟甲基)苯基]苯磺酰胺 phosphate m 3M3FBS Inhibitor m3M3FBS leukemic U937 inositol inhibit neutrophils m-3M-3FBS inhibitor