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Scriptaid

Scriptaid

产品编号 T6980   CAS 287383-59-9
别名: GCK1026, Scriptide

Scriptaid (GCK1026) 是一种组蛋白去乙酰化酶抑制剂,也是抗病毒药的敏化剂。它可研究癌症,与爱泼斯坦-巴尔病毒相关的淋巴瘤。

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Scriptaid Chemical Structure
Scriptaid, CAS 287383-59-9
规格 价格/CNY 货期 数量
1 mg ¥ 219 现货
5 mg ¥ 498 现货
10 mg ¥ 898 现货
25 mg ¥ 1,910 现货
50 mg ¥ 3,570 现货
100 mg ¥ 5,160 现货
500 mg ¥ 10,800 现货
1 mL * 10 mM (in DMSO) ¥ 562 现货
产品目录号及名称: Scriptaid (T6980)
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纯度: 99.91%
纯度: 98.05%
纯度: 96.74%
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Scriptaid (GCK1026) is an inhibitor of HDAC, and has a greater effect on acetylated H4 than H3.
体外活性 Scriptaid (6 μM) results in a >100-fold increase in histone acetylation in PANC-1 cell. Scriptaid (8 μM) is not lethal to PANC-1 cell and has limited effects (80% survival) on MDAMB-468. Scriptaid increases the transcription of pCMVb, p6SBE-luc and p6MBE-luc independent of a positive inducer of transcription. Scriptaid is capable of inducing high expression of p6MBE-luc, pCMVb, and pUB6/V5-LacZ, driven by viral (SV40 and CMV) or human (ubiquitin c, UB6) promoters, which do not depend on the specificity of the enhancer (SBE versus MBE), the type of promoter (viral versus cellular), the product of the reporter gene (luciferase versus b-gal), nor on the integration status of the reporter construct. [1] Scriptaid induces high rates of somatic cell nuclear transfer (SCNT) oocytes development to the blastocyst stage and allowed full-term development (3.4, 4.2, 7.6, 6.8, and 4.1%) with all concentrations (50, 100, 250, 500, and 2000 nM respectively). Scriptaid improves the full-term development of cloned B6D2F1embryos in a dose-dependent manner with the maximum effect at 250 nM. Scriptaid enables the clone of all the important inbred mouse strains, such as C57BL/6, C3H/He, DBA/2, and 129/Sv. Scriptaid treatment enhances newly synthesized mRNA levels in cloned embryos. 250 nM Scriptaid treated for up to 48 h, does not inhibit the development of ICSI-fertilized embryos. [2] Scriptaid inhibits T. gondii tachyzoite proliferation with IC50 of 39 nM. Scriptaid (0.225 μM) completely protects the HS68 monolayers from T. gondii tachyzoite. [3] Scriptaid inhibits growth of ER negative cell lines, MDA-MB-231, MDA-MB-435 and Hs578t with IC50 of 0.5-1.0 μg/mL after 48 h treatment. 1 μg/ml Scriptaid treated for 48 h induces an accumulation of both acetylated H3 and acetylated H4 histone tail proteins, and a maximal of 20,000-fold increase of ER mRNA transcript. [4]
体内活性 Scriptaid elicits a dose-dependent decrease in lesion size (a maximal decrease of 45%) at 1.5 to 5.5 mg/kg and a concomitant attenuation in motor and cognitive deficits when delivered 30 minutes postinjury in a model of mode rate TBI. Comparable protection is achieved even when treatment is delayed to 12 h postinjury. The protection of motor and cognitive functions is long lasting, as similar improvements are detected 35 days postinjury. Scriptaid induces an increase in surviving neurons (42%), as well as the number/length of their processes within the CA3 region of the hippocampus and the pericontusional cortex. Scriptaid treatment prevents the decrease in phospho-AKT (p-AKT) and phosphorylated phosphatase and tensin homolog deleted on chromosome 10 ( p-PTEN) induced by TBI in cortical and CA3 hippocampal neurons. [6] Scriptaid treatment (3.5 mg/kg) clearly inhibits tumor growth in a human breast cancer xenograft MDA-MB-231 model, reducing tumor volume by 75%. [4]
激酶实验 Immunoblotting assay of histone acetylation: PANC-1 cells are treated with 2 μg/mL of Scriptaid for 18 h in culture medium. Treated and untreated cells are harvested with trypsin-EDTA, washed with PBS, and resuspended in a protein sample buffer. Protein concentration is determined by BCA protein assay reagents. Fifty μg of proteins from each sample is loaded on a 12% denaturing polyacrylamide gel. Proteins are subsequently transferred to a nylon membrane using MilliblotGraphite Electroblotter I. The nylon membrane is incubated with rabbit antihuman acetyl-lysine antibody, followed by goat antirabbit antibody coupled to horseradish peroxidase, developed with SuperSignal substrates, and detected by film.
细胞实验 Cells are plated at a cell density of 5000 cells/well in 12 well plates and treated with Scriptaid for up to 3 days. Cells numbers are counted daily using a Coulter counter.(Only for Reference)
别名 GCK1026, Scriptide
分子量 326.35
分子式 C18H18N2O4
CAS No. 287383-59-9

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 32.6 mg/mL (100 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0642 mL 15.321 mL 30.6419 mL 76.6049 mL
5 mM 0.6128 mL 3.0642 mL 6.1284 mL 15.321 mL
10 mM 0.3064 mL 1.5321 mL 3.0642 mL 7.6605 mL
20 mM 0.1532 mL 0.766 mL 1.5321 mL 3.8302 mL
50 mM 0.0613 mL 0.3064 mL 0.6128 mL 1.5321 mL
100 mM 0.0306 mL 0.1532 mL 0.3064 mL 0.766 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Su GH, et al. Cancer Res, 2000, 60(12), 3137-3142. 2. Van Thuan N, et al. Reproduction, 2009, 138(2), 309-317. 3. Strobl JS, et al. J Parasitol, 2007, 93(3), 694-700. 4. Keen JC, et al. Breast Cancer Res Treat, 2003, 81(3), 177-186. 5. Takai N, et al. Int J Mol Med, 2006, 17(2), 323-329. 6. Janaki Ramaiah M, et al. Scriptaid cause histone deacetylase inhibition and cell cycle arrest in HeLa cancer cells: A study on structural and functional aspects. Gene. 2017 Sep 5;627:379-386.
Suberoyl bis-hydroxamic acid HDAC-IN-51 SantacruzaMate A Splitomicin SAHA-OH PTACH MC2590 HDACs/mTOR Inhibitor 1

相关化合物库

该产品包含在如下化合物库中:
血液病分子库 DNA 损伤和修复分子库 抗癌化合物库 抗病毒库 组蛋白修饰化合物库 已知活性化合物库 抗胰腺癌化合物库 抑制剂库 免疫/炎症分子化合物库 抗乳腺癌化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Scriptaid 287383-59-9 Apoptosis Autophagy Chromatin/Epigenetic DNA Damage/DNA Repair Microbiology/Virology HDAC Influenza Virus GCK-1026 GCK1026 Scriptide Inhibitor GCK 1026 Histone deacetylases inhibit inhibitor

 

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