Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Bestatin (Ubenimex) 是 CD13 (Aminopeptidase N)/APN 和 leukotriene A4 hydrolase 抑制剂,可用于癌症研究。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 182 | 现货 | ||
5 mg | ¥ 369 | 现货 | ||
10 mg | ¥ 593 | 现货 | ||
25 mg | ¥ 995 | 现货 | ||
50 mg | ¥ 1,690 | 现货 | ||
100 mg | ¥ 2,920 | 现货 | ||
500 mg | ¥ 6,650 | 现货 |
产品描述 | Bestatin (Ubenimex) competitively inhibits many aminopeptidases, including B, N and leucine aminopeptidases. Ubenimex is a microbial metabolite and dipeptide with potential immunomodulatory and antitumor activities. Aminopeptidases has been implicated in the process of cell adhesion and invasion of tumor cells. Therefore, inhibiting aminopeptidases may partially attribute to the antitumor effect of ubenimex. This agent also activates T lymphocyte, macrophage, and bone marrow stem cell as well as stimulates the release of interleukin-1 and -2, thus further enhances its antitumor activity. |
体外活性 | 在B16-BL6黑色素瘤细胞荷瘤的小鼠背部侧中,Bestatin使既定原发性肿瘤块血管的数量下降.在小鼠背部气囊测定中,Bestatin显著抑制黑色素瘤细胞诱导的血管生成. 在EGDA大鼠中,Bestatin可降低EAC的发病率(57.7%降至26.1%).在EGDA大鼠的食管组织中,Bestatin对白三烯B4生物合成的抑制具有明显的统计学意义. |
体内活性 | Bestatin对SN12M细胞侵入重组基底膜(基质胶)具有浓度依赖性的抑制作用。在非肿瘤条件培养基中,其还可浓度依赖性抑制肿瘤细胞IV型胶原退化。在U937细胞中,乌苯美司可使caspase-3的活性增强并诱导DNA梯和DNA片段化。在SN12M Bestatin抑制氨基肽酶底物的水解活性。Bestatin对人脐静脉内皮细胞中的管状形成具有抑制作用。通过固定在细胞表面的亮氨酸氨肽酶,Bestatin直接刺激淋巴细胞(和单核细胞),而间接刺激则是通过氨基肽酶乙抑制吞噬作用激素的分解代谢发挥。 |
激酶实验 | Cells are harvested, washed, and lysed in NP-40 lysis buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.5% NP-40). Total cell protein is quantified using the Bradford assay and 1-mg/mL protein aliquots are made. Ten microliters of total cell protein is mixed with 290 μL of substrate solution (0.1 mg/mL dithiothreitol [DTT], 0.1 mg/mL albumin, and 1 mM alanine-β-naphthylamide). Fluorometric measurements (340 nm excitation, 400 nm emission) are made after 15 and 30 min. The slope of the line between the 15- and 30-min measurements is used to represent aminopeptidase activity. Total cell protein is preincubated with bestatin, amastatin, puromycin, EDTA, and/or ZnCl2 for 20 min before the fluorometric aminopeptidase assay. |
细胞实验 | Growing cells (1×106 to 2×106 cells/mL) are diluted to 1.0×103 cells/mL and transferred (3 mL) into a well in a 12-well multiwell plate (2.5-cm diameter/well). Cells are treated with 0, 10, 50, 100, 300, or 600 μM Bestatin and allowed to grow at 21°C shaking at 180 rpm for 48 h. A hemocytometer is used to measure cell density after 0, 24, and 48 h. |
别名 | Ubenimex, 乌苯美司 |
分子量 | 308.37 |
分子式 | C16H24N2O4 |
CAS No. | 58970-76-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
1eq. NaOH: 15.4 mg/mL (50 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
1eq. NaOH | 1 mM | 3.2429 mL | 16.2143 mL | 32.4286 mL | 81.0714 mL |
5 mM | 0.6486 mL | 3.2429 mL | 6.4857 mL | 16.2143 mL | |
10 mM | 0.3243 mL | 1.6214 mL | 3.2429 mL | 8.1071 mL | |
20 mM | 0.1621 mL | 0.8107 mL | 1.6214 mL | 4.0536 mL | |
50 mM | 0.0649 mL | 0.3243 mL | 0.6486 mL | 1.6214 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Bestatin 58970-76-6 Immunology/Inflammation Metabolism Microbiology/Virology Others Aminopeptidase Antibacterial Antibiotic LTR Bacterial Inhibitor Ubenimex 乌苯美司 inhibit inhibitor