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Amifostine thiol dihydrochloride

产品编号 TQ0133 CAS 14653-77-1

别名: WR 1065, 硫代乙基氨基乙基胺盐酸盐

Amifostine thiol dihydrochloride (WR 1065) 可通过 JNK 依赖性信号通路激活 p53。它还可以保护正常组织免受某些抗癌药物的毒性作用。

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Amifostine thiol dihydrochloride Chemical Structure

Amifostine thiol dihydrochloride, CAS 14653-77-1

规格	价格/CNY	货期
1 mg	￥ 195	现货
5 mg	￥ 462	现货
10 mg	￥ 756	现货
25 mg	￥ 1,251	现货
50 mg	￥ 1,856	现货
100 mg	￥ 2,778	现货
1 mL * 10 mM (in DMSO)	￥ 528	现货

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其他形式的 Amifostine thiol dihydrochloride:

Amifostine thiol
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纯度: 100%

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生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

产品描述	Amifostine thiol dihydrochloride (WR 1065) can activate p53 through a JNK-dependent signaling pathway. It also protects normal tissues from the toxic effects of certain cancer drugs.
体外活性	The DNA-binding activity is increased in a WR-1065 concentration-dependent manner. Cells treated with 1 mM WR-1065 dihydrochloride for 24 h revealed that all of the p53-induced genes analyzed are transactivated following WR-1065 dihydrochloride treatment, in a p53-dependent manner. Significantly, treatment with WR-1065 dihydrochloride leads to a 3-fold increase in luciferase expression driven by AP-1 and a 5-fold increase when this reporter gene is driven by NF-κB when these values are normalized to the level of the cotransfected β-galactosidase gene [2].
体内活性	WR-1065 attenuates the severity of 6-OHDA-induced catalepsy when compared with 6-OHDA-lesioned rats. Also, it has been observed that WR-1065 dihydrochloride improves catalepsy in a dose-dependent manner. Pretreatment with three different doses of WR-1065 dihydrochloride (20, 40, and 80 μg/2 μL/rat) for 3 days before 6-OHDA administration, significantly elevates SOD activity and restores it to normal range compare with 6-OHDA lesioned rats [3].
细胞实验	For Western analysis, cells are treated with 1 mM WR-1065 for 24 h, and subconfluent cultures of cells are harvested and lysed in RIPA buffer supplemented with protease inhibitors. Protein concentrations are determined by a detergent-compatible assay. Western blots are blocked and incubated in antibody in PBS/0.2% Tween 20/5% nonfat dry milk. Blots are incubated with 1 μg/mL antibody for 1 h at room temperature, followed by washing in PBS/0.2% Tween 20 and incubation in peroxidase-conjugated secondary antibody and chemiluminescence detection [2].
动物实验	Seventy-two rats are divided randomly into 9 equal groups: 1) Control group receives no injection and is left untreated for the entire period of the experiment as intact animals; 2) Sham-operated group is subjected only to surgical procedure; 3) Vehicle (saline)-treated group receives 2 μL saline (Intra-SNc); 4) Lesioned group receives 6-hydroxydopamine; 5) Vehicle+6OHDA group receives saline as a vehicle 3 days once daily (2 μL/rat) before 6-OHDA injection; 6 to 8) Rats in these groups are pretreated with Intra-SNc injection of WR-1065 (20, 40 and 80 μg/2 μL/rat) 3 days before 6-OHDA injection; 9) Non-lesioned animals receive intra-SNc injection of WR-1065 dihydrochloride (80 μg/2 μL/rat) for three days [3].

别名	WR 1065, 硫代乙基氨基乙基胺盐酸盐
分子量	207.165
分子式	C5H16Cl2N2S
CAS No.	14653-77-1

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 24 mg/mL (115.85 mM), Sonication is recommended.

H2O: 100 mg/mL (482.72 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO / H2O	1 mM	4.8271 mL	24.1354 mL	48.2707 mL	120.6768 mL
	5 mM	0.9654 mL	4.8271 mL	9.6541 mL	24.1354 mL
	10 mM	0.4827 mL	2.4135 mL	4.8271 mL	12.0677 mL
	20 mM	0.2414 mL	1.2068 mL	2.4135 mL	6.0338 mL
	50 mM	0.0965 mL	0.4827 mL	0.9654 mL	2.4135 mL
	100 mM	0.0483 mL	0.2414 mL	0.4827 mL	1.2068 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

溶液体积

质量

配液浓度

参考文献

1. Pluquet O, et al. The cytoprotective aminothiol WR1065 activates p53 through a non-genotoxic signaling pathway involving c-Jun N-terminal kinase. J Biol Chem. 2003 Apr 4;278(14):11879-87. 2. Shen H, et al. Binding of the aminothiol WR-1065 to transcription factors influences cellular response to anticancer drugs. J Pharmacol Exp Ther. 2001 Jun;297(3):1067-73. 3. Afshin Kheradmand, et al. Effect of WR-1065 on 6-hydroxydopamine-induced catalepsy and IL-6 level in rats. Iran J Basic Med Sci. 2016 May; 19(5): 490-496.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Amifostine thiol dihydrochloride 14653-77-1 Apoptosis p53 Amifostine thiol WR-1065 Inhibitor MDM-2/p53 Amifostine thiol Dihydrochloride WR 1065 WR1065 inhibit 硫代乙基氨基乙基胺盐酸盐 inhibitor

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Amifostine thiol dihydrochloride

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords