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1-Aminobenzotriazole

产品编号 T5953 CAS 1614-12-6

别名: 3-Aminobenzotriazole, 1-氨基苯并三唑, ABT

1-Aminobenzotriazole (ABT) 是一种不可逆的细胞色素 P450 非特异性抑制剂。

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1-Aminobenzotriazole, CAS 1614-12-6

规格	价格/CNY	货期
25 mg	￥ 292	现货
50 mg	￥ 410	现货
100 mg	￥ 497	现货
200 mg	￥ 671	现货
500 mg	￥ 1,347	现货
1 mL * 10 mM (in DMSO)	￥ 460	现货

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生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

产品描述	1-Aminobenzotriazole (ABT) (ABT) is a non-specific cytochrome P450 (CYP) inhibitor.
体外活性	In vitro, ABT rapidly and efficiently destroyed P450 in both hepatic and renal microsomes prepared from naive male Sprague-Dawley rats.?Incubation of hepatic or renal microsomes in vitro with ABT produced detectable destruction of P450 within 5 min. Maximal destruction of P450 occurred within 10 min in both hepatic and renal microsomes during in vitro incubation with ABT. ABT-induced destruction of P450 in vitro was concentration-dependent.?For hepatic microsomes, maximal destruction of about 70% of P450 required concentrations of ABT equal to or greater than 10 mM. For renal microsomes, maximal destruction of about 80% of P450 required concentrations of ABT equal to or greater than 10 mM. In both liver and kidney, only P450 content and P450-dependent activities were significantly decreased[1].
体内活性	Hepatic and renal microsomes and cytosol were prepared from male Sprague-Dawley rats following ABT pretreatment (0-100 mg/kg ip) for various times.?Administration of 100 mg ABT/kg produced profound reductions in P450 content in both liver and kidney within 2 hr;?loss of P450 in both tissues persisted for at least 48 hours.?ABT-induced destruction of P450 was dose-dependent.?Maximal destruction of about 80% of total hepatic P450 occurred at dosages of ABT equal to or greater than 10 mg/kg.?Maximal destruction of about 80% of total renal P450 occurred at dosages of ABT equal to or greater than 50 mg/kg[1].
细胞实验	Animals were killed by cervical dislocation and decapitation.?Kidneys and livers were excised quickly and placed in ice cold 1.15% KC].?Renal inner medulla and papilla were discarded.?Renal cortex and liver were minced and homogenized in 3 vol of 100 mM phosphate buffer (pH 7.4) containing 250 mM sucrose and 1.5 mM EDTA.?Kidney and liver homogenates were centrifuged at 10000g for 20 min. The resulting supematant was centrifuged at 105000g for 60 min. The 105000g supematant (cytosol) was used for the determination of glutathione S-transferase activity .?The microsomal fraction (pellet) was resuspended in phosphate buffered sucrose (pH 7.4) and centrifuged at 105000g for 60 min. The resulting microsomal fraction was resuspended in phosphate-buffered sucrose (pH 7.4) containing 20% glycerol to a final concentration of l0-20 mg protein per milliliter.?The microsomal fraction was used for the determination of P450, cytochrome b5, and NADPH-cytochrome-c reductase activities.?Microsomes from control or ABT-treated rats were either analyzed on the day of preparation or stored overnight at -80 C. In all cases, control microsomes were handled identically with ABT-treated microsomes.?P450 content in control and ABT-treated microsomes was not affected by overnight storage[1].
动物实验	For in vivo experiments, rats received ABT (O-100 mg/kg) dissolved in normal saline at a concentration of I-50 mg/ml.?ABT was administered ip and rats were killed at various times thereafter.?Total injection volume was either 1 or 2 ml/kg and control rats received 2 ml saline/kg[1].

别名	3-Aminobenzotriazole, 1-氨基苯并三唑, ABT
分子量	134.14
分子式	C6H6N4
CAS No.	1614-12-6

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 125 mg/mL (931.86 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	7.4549 mL	37.2745 mL	74.549 mL	186.3724 mL
	5 mM	1.491 mL	7.4549 mL	14.9098 mL	37.2745 mL
	10 mM	0.7455 mL	3.7274 mL	7.4549 mL	18.6372 mL
	20 mM	0.3727 mL	1.8637 mL	3.7274 mL	9.3186 mL
	50 mM	0.1491 mL	0.7455 mL	1.491 mL	3.7274 mL
	100 mM	0.0745 mL	0.3727 mL	0.7455 mL	1.8637 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

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分子量

浓度 (起始)

容积 (起始)

浓度 (终)

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参考文献

1. Mugford C A , Mortillo M , Mico B A , et al. 1-Aminobenzotriazole-Induced Destruction of Hepatic and Renal Cytochromes P450 in Male Sprague-Dawley Rats[J]. Fundamental and Applied Toxicology, 1992, 19(1):43-49. 2. Yang K , Hye Koh K , Jeong H . Induction of CYP2B6 and CYP3A4 Expression by 1-Aminobenzotriazole (ABT) in Human Hepatocytes[J]. Drug Metabolism Letters, 2010, 4(3):129-133.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

1-Aminobenzotriazole 1614-12-6 Metabolism P450 inhibit Cytochrome P450 3-Aminobenzotriazole 1-氨基苯并三唑 ABT 1Aminobenzotriazole 1 Aminobenzotriazole Inhibitor CYPs inhibitor

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1-Aminobenzotriazole

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords