Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Hydrochlorothiazide (HCTZ) 是一种口服有效的噻嗪类的利尿药,可抑制转化 TGF-β/Smad 信号通路。它通过打开钙激活钾 (KCA) 通道发挥直接的血管松弛作用。它改善心脏功能,减少纤维化并具有降压作用。
产品描述 | Hydrochlorothiazide (HCTZ) is a Thiazide Diuretic. The physiologic effect of hydrochlorothiazide is by means of Increased Diuresis. |
激酶实验 | Kinase autophosphorylation assays: Kinase assays using wild-type and mutant glutathione S-transferase (GST)-Abl fusion proteins (c-Abl amino acids 220-498) are done. GST-Abl fusion proteins are released from glutathione-Sepharose beads before use; the concentration of ATP is 5 μM. Immediately before use in kinase autophosphorylation and in vitro peptide substrate phosphorylation assays, GST-Abl kinase domain fusion proteins are treated with LAR tyrosine phosphatase. After 1-hour incubation at 30 °C, LAR phosphatase is inactivated by addition of sodium vanadate (1 mM). Immunoblot analysis comparing untreated GST-Abl kinase to dephosphorylated GST-Abl kinase is routinely done using phosphotyrosine-specific antibody 4 g10 to confirm complete (>95%) dephosphorylation of tyrosine residues and c-Abl antibody CST 2862 to confirm equal loading of GST-Abl kinase. The Dasatinib concentration range is extended to 1,000 nM for mutant T315I. These same inhibitor concentrations are used for the in vitro peptide substrate phosphorylation assays. The three inhibitors are tested over these same concentration ranges against GST-Src kinase and GST-Lyn kinase. |
别名 | HCTZ, 双氢氯噻嗪, 氢氯噻嗪 |
分子量 | 297.74 |
分子式 | C7H8ClN3O4S2 |
CAS No. | 58-93-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 20 mg/mL(67.2 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.3586 mL | 16.7932 mL | 33.5864 mL | 83.9659 mL |
5 mM | 0.6717 mL | 3.3586 mL | 6.7173 mL | 16.7932 mL | |
10 mM | 0.3359 mL | 1.6793 mL | 3.3586 mL | 8.3966 mL | |
20 mM | 0.1679 mL | 0.8397 mL | 1.6793 mL | 4.1983 mL | |
50 mM | 0.0672 mL | 0.3359 mL | 0.6717 mL | 1.6793 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Hydrochlorothiazide 58-93-5 Membrane transporter/Ion channel Metabolism Stem Cells Potassium Channel Carbonic Anhydrase TGF-beta/Smad relaxant HCTZ 双氢氯噻嗪 calcium KcsA fibrosis potassium vascular antihypertensive KCA Transforming growth factor beta inhibit 氢氯噻嗪 cardiac Inhibitor inhibitor