Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NOD-IN-1 (Compound 4) 是核苷酸结合寡聚化结构域样受体 NOD1(IC50:5.74 μM)和 NOD2 (IC50:6.45 μM)的抑制剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 623 | 现货 | ||
5 mg | ¥ 1,230 | 现货 | ||
10 mg | ¥ 1,960 | 现货 | ||
25 mg | ¥ 3,870 | 现货 | ||
50 mg | ¥ 5,550 | 现货 | ||
100 mg | ¥ 7,770 | 现货 | ||
500 mg | ¥ 15,500 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 938 | 现货 |
产品描述 | NOD-IN-1 (Compound 4) is a potent mixed inhibitor of nucleotide-binding oligomerization domain (NOD)-like receptors. |
靶点活性 | NOD2:6.45 μM, NOD1:5.74 μM |
体外活性 | NOD-IN-1 (compound 4) is potent mixed inhibitor of NOD1 and NOD2, displaying a balanced inhibitory activity on both targets in the low micromolar range. NOD-IN-1 (IC50 (NOD1)=5.74 μM; IC50 (NOD2)=6.45 μM) is identified as the best of the series, possessing NOD1- and NOD2-inhibitory activities in the lower micromolar range. These results show that NOD-IN-1 is 7-fold less potent than Noditinib-1 in terms of NOD1 inhibition and completely devoid of selective activity for NOD1 or NOD2 as opposed to Noditinib-1. NOD-IN-1 exhibits balanced dual activities of less than 10 μM on the two targets[1]. |
细胞实验 | NOD-IN-1 is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. An MTS assay in which the proliferation rates of HEK-Blue NOD1 cells are measured in the presence of Noditinib-1 and of the synthesized potential NOD1 inhibitor NOD-IN-1 is employed to screen these compounds for potential cytotoxicity. Cells are treated for 24 h with the compound of interest at concentrations of up to 25 μM. Comparison of the resulting metabolic activities with that of the untreated control showed that all compounds are well tolerated by HEK-Blue NOD1 cells, since their residual metabolic activities do not fall below 80% at the maximum concentration tested[1]. |
别名 | Compound 4 |
分子量 | 343.4 |
分子式 | C18H17NO4S |
CAS No. | 132819-92-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/mL (160.16 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.9121 mL | 14.5603 mL | 29.1206 mL | 72.8014 mL |
5 mM | 0.5824 mL | 2.9121 mL | 5.8241 mL | 14.5603 mL | |
10 mM | 0.2912 mL | 1.456 mL | 2.9121 mL | 7.2801 mL | |
20 mM | 0.1456 mL | 0.728 mL | 1.456 mL | 3.6401 mL | |
50 mM | 0.0582 mL | 0.2912 mL | 0.5824 mL | 1.456 mL | |
100 mM | 0.0291 mL | 0.1456 mL | 0.2912 mL | 0.728 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NOD-IN-1 132819-92-2 Immunology/Inflammation NF-Κb NOD-like Receptor (NLR) NOD NODIN1 Compound 4 inhibit NOD IN 1 Inhibitor inhibitor