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BV6

BV6

产品编号 T6428   CAS 1001600-56-1

BV6 是 c-IAP1 和 XIAP 的拮抗剂,它们是凋亡抑制剂 (IAP) 家族的成员。

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BV6 Chemical Structure
BV6, CAS 1001600-56-1
规格 价格/CNY 货期 数量
1 mg ¥ 373 现货
2 mg ¥ 497 现货
5 mg ¥ 675 现货
10 mg ¥ 1,180 现货
25 mg ¥ 2,310 现货
50 mg ¥ 3,430 现货
100 mg ¥ 4,990 现货
1 mL * 10 mM (in DMSO) ¥ 1,580 现货
产品目录号及名称: BV6 (T6428)
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纯度: 99.89%
纯度: 99.46%
纯度: 98%
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 BV6 is an antagonist of c-IAP1 and XIAP, members of the inhibitors of apoptosis (IAP) family.
体外活性 Treatment of HCC193 cells with 1 μM BV6 for 24 hours causes a significant survival curve shift in HCC193 cells relative to DMSO-treated cells, with a DER=1.38 (p<0.05). BV6 (2 and 5 μM) significantly represses BrdU incorporation in ectopic and eutopic (disease-free and myomas) ESCs. An ~30% decrease of BrdU incorporation is observed in both groups after treatment with 5 μM BV6. Administration of 1 μM BV6 to HCC193 cells induces complete depletion of cIAP1 levels at 1 hour post-treatment, while a decrease in XIAP levels is not seen until 24 hours following addition of drug. Similarly, 5 μM BV6 fully depletes c-IAP1 at 1 hour and begin to reduce XIAP at 24 hours in H460 cells. In parallel findings, c-IAP1 levels are decreased in response to a small dose of 0.25 μM BV6 in both cell lines, whereas trace amounts of XIAP are still present at 5 μM BV6. HCC193 cells demonstrates noticeable cleaved caspase-3 levels beginning 12 hours post-incubation with 1 μM BV6, and cleaved caspase-3 levels continued to increase in a time-dependent manner over 48 hours.
体内活性 Murine c-IAP-1, c-IAP-2 and XIAP expressions are clearly observed in the cytoplasm of both epithelial and stromal cells of implants, whereas Survivin is mainly expressed in the nuclei BV6 treatment for 4 weeks attenuated the intensity of IAPs expression. After immunohistochemical staining, cytokeratin and vimentin are positively stained, whereas calretinin is negative. After BV6 treatment for 4 weeks, the total number of lesions (4.6 versus 2.8/mouse), the average weight (78.1 versus 32.0 mg/mouse) and the surface area (44.5 versus 24.6 mm2/mouse) of lesions are significantly less than in the controls. In the endometrial gland epithelia or stroma, the percentage of Ki67-positive cells decreases after BV6 treatment.
细胞实验 BV6 is prepared in DMSO and stored, and then diluted with appropriate medium before use. H460 and HCC193 cell lines are cultured in RPMI-1640 supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Cell viability is measured using the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay kit. 5000 cells/well are seeded into 96-well plates in triplicate. Following adhesion of cells to the wells, increasing concentrations of BV6 are added into different wells. Control groups are exposed to the same concentration of DMSO. The final concentrations of 333 μg/mL MTS and 25 μM PMS are added to each well 24 hours later. After two hours incubation at 37°C in humidified 5% CO2, plates are read at the absorbance of 490 nm on a microplate reader. Relative cell viability of an individual sample is calculated by normalizing their absorbance to that of the corresponding control. IC50 values are calculated using Prism 5.01. For the TNFα neutralizing antibody assay, cells are exposed to 1 and 5 μM BV6 with or without 10 μg/mL Infliximab and the assay is performed 24 hours later. Plates are read at the absorbance of 490 nm on a microplate reader.
动物实验 BV6 is prepared in DMSO and diluted with saline or PBS. Female mice (6 weeks of age, BALB/c) are used. All 24 mice are ovariectomized through a 1 cm longitudinal skin incision then injected s.c. with estradiol valerate (0.5 μg/mouse/week) once per week for 6 weeks until the experimental endometriosis induction. Two weeks after ovariectomy, the uteri of an additional eight donor mice (n=8) are removed en bloc after euthanasia and cleaned of excess tissue in sterile saline. Each uterus is cut to include the uterine horns in each half with a linear incision longitudinally and minced (0.5 mm in diameter) with dissecting scissors. The ovariectomized recipient mice (n=16) are anesthetized using pentobarbital sodium. A 0.5 cm subabdominal midline incision is made. Each recipient receives half of the donor uterus (1:2 donor uterus to host ratio) minced and added to 500 μl saline, and injected into the peritoneal cavity, and the peritoneum is sutured. Injected uterine tissue weighed ~50 mg per mouse. For the next 4 weeks, recipient mice are treated with a single i.p. injection of BV6 (n=8; 10 mg/kg) or vehicle (n=8; 1% DMSO) twice weekly.
分子量 1205.57
分子式 C70H96N10O8
CAS No. 1001600-56-1

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 55 mg/mL (45.62 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.8295 mL 4.1474 mL 8.2948 mL 20.7371 mL
5 mM 0.1659 mL 0.8295 mL 1.659 mL 4.1474 mL
10 mM 0.0829 mL 0.4147 mL 0.8295 mL 2.0737 mL
20 mM 0.0415 mL 0.2074 mL 0.4147 mL 1.0369 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Li W, et al. J Thorac Oncol. 2011, 6(11), 1801-1809. 2. Müller-Sienerth N, et al. PLoS One. 2011, 6(6), e21556 3. Rettinger E, et al. Front Pediatr. 2014, 18, 2:75. 4. Uegaki T, et al. Inhibitor of apoptosis proteins (IAPs) may be effective therapeutic targets for treating endometriosis. Hum Reprod. 2015 Jan;30(1):149-58. 5. Xu H, Tang Z, Zuo Y, et al. Molecular dynamics simulation revealed the intrinsic conformational change of cellular inhibitor of apoptosis protein-1[J]. Journal of Biomolecular Structure and Dynamics. 2020, 38(4): 975-984.

文献引用

1. Xu H, Tang Z, Zuo Y, et al. Molecular dynamics simulation revealed the intrinsic conformational change of cellular inhibitor of apoptosis protein-1. Journal of Biomolecular Structure and Dynamics. 2020, 38(4): 975-984 2. Li Y, Lee H H, Jiang V C, et al.Potentiation of apoptosis in drug-resistant mantle cell lymphoma cells by MCL-1 inhibitor involves downregulation of inhibitor of apoptosis proteins.Cell Death & Disease.2023, 14(11): 714.
Xevinapant Embelin LCL161 Se-Methylselenocysteine SM-164 Hydrochloride (957135-43-2 free base) SM-164 Anticancer agent 128 MX69

相关化合物库

该产品包含在如下化合物库中:
NO PAINS 化合物库 经典已知活性库 抑制剂库 PPI抑制剂库 细胞凋亡化合物库 已知活性化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

BV6 1001600-56-1 Apoptosis IAP inhibit Inhibitor BV 6 BV-6 inhibitor

 

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