Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Angiotensin (1-7) (ASP-ARG-VAL-TYR-ILE-HIS-PRO) 是肾素-血管紧张素系统中的内源性七肽。Angiotensin 1-7在心肌细胞中具有抗炎和抗纤维化活性,因此具有心脏保护作用。它能抑制血管紧张素转换酶和释放一氧化氮,是激肽诱导的血管舒张的局部协同调节剂。它阻断 Ang II 诱导的平滑肌细胞增殖和肥大,并显示对内皮的抗血管生成和生长抑制作用。它显示出抗炎活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 243 | 现货 | ||
5 mg | ¥ 428 | 现货 | ||
10 mg | ¥ 672 | 现货 | ||
25 mg | ¥ 1,490 | 现货 | ||
50 mg | ¥ 2,370 | 现货 | ||
100 mg | ¥ 3,490 | 现货 |
产品描述 | Angiotensin (1-7) (ASP-ARG-VAL-TYR-ILE-HIS-PRO) is a synthetic heptapeptide identical to endogenous angiotensin-(1-7) , inhibits purified canine ACE activity (IC50=0.65 μM) with vasodilator and antiproliferative activities. |
靶点活性 | ACE:0.65 μM (IC50) |
体外活性 | ANG-(1-7) significantly attenuated ANG II-induced cardiac hypertrophy and perivascular fibrosis in these rats. These effects of ANG-(1-7) were confirmed in cultured cardiomyocytes, in which ANG-(1-7) significantly attenuated ANG II-induced increases in cell size[1]. |
体内活性 | Ang-(1-7) treatment attenuated the shortening of the atrial effective refractory period and the increase in the atrial fibrillation inducibility level. Immunohistochemistry and Western blotting showed sympathetic nerve hyperinnervation in the pacing group, while Ang-(1-7) attenuated sympathetic nerve proliferation. Ang-(1-7) alleviated the pacing-induced increases in tyrosine hydroxylase and nerve growth factor mRNA expression levels[2]. |
细胞实验 | Cultured cardiomyocytes were treated with PBS (control), ANG II (100 nM), ANG-(1–7) (100 nM), ANG II + ANG-(1–7), ANG II + ANG-(1–7) + A779 (10 μM), ANG II + ANG-(1–7) + Sirt3 short hairpin (sh)RNA, lentiviral particles (1 × 10^6 TU) containing a Sirt3 shRNA viral vector, or ANG II + Mito-TEMPO (0.1 μM) for 24 h. Each treatment was performed in triplicate wells. Monoclonal anti-α-sarcomeric actin antibody was used for identification and measurement of cardiomyocytes via immunofluorescence staining. Cells were fixed in methanol at ?10°C and washed with fresh washing solution (0.1% Triton X-100 in PBS) three times. After preincubation with 3% BSA for 20 min, cells were incubated with primary antibody, which was diluted to 1:100 with PBS containing 3% BSA, overnight at 4°C. After three washings with the washing solution, cells were incubated with fluorescence-conjugated anti-mouse IgG (1:500 diluted in PBS) for 1 h at room temperature. Photographic images of cardiomyocytes were taken with a fluorescence microscope. Image-analysis software was used to measure the cell surface area of cardiomyocytes that were positively stained for sarcomeric actin. Variations in cell size were expressed as relative cell surface area versus the control. At least 50 cells in each dish were randomly selected for surface area analysis[1]. |
动物实验 | Eighteen dogs were randomly assigned to sham group, pacing group and Ang-(1-7) group. Rapid atrial pacing was maintained for 14 days in the pacing and Ang-(1-7) groups. Ang-(1-7) was administered intravenously in the Ang-(1-7) group. The atrial effective refractory period and atrial fibrillation inducibility level were measured at baseline and under sympathetic nerve stimulation after 14 days of measurement. The atrial sympathetic nerves labeled with tyrosine hydroxylase were detected using immunohistochemistry and Western blotting, and tyrosine hydroxylase and nerve growth factor mRNA levels were measured by reverse transcription polymerase chain reaction[2]. |
别名 | Ang-(1-7), Angiotensin 1-7, ASP-ARG-VAL-TYR-ILE-HIS-PRO |
分子量 | 899 |
分子式 | C41H62N12O11 |
CAS No. | 51833-78-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 8.99 mg/ml (10 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 1.1123 mL | 5.5617 mL | 11.1235 mL | 27.8087 mL |
5 mM | 0.2225 mL | 1.1123 mL | 2.2247 mL | 5.5617 mL | |
10 mM | 0.1112 mL | 0.5562 mL | 1.1123 mL | 2.7809 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Angiotensin (1-7) 51833-78-4 Endocrinology/Hormones Metabolism RAAS Endogenous Metabolite Angiotensin Receptor Angiotensin (17) Ang Inhibitor Ang-(1-7) inhibit Angiotensin 1-7 Angiotensin ASP-ARG-VAL-TYR-ILE-HIS-PRO Angiotensin (1 7) Angiotensin-converting Enzyme (ACE) inhibitor