Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) 是一种 Caspase-3底物。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 375 | 现货 | ||
5 mg | ¥ 1,180 | 现货 | ||
10 mg | ¥ 1,770 | 现货 | ||
25 mg | ¥ 3,130 | 现货 | ||
50 mg | ¥ 4,480 | 现货 | ||
100 mg | ¥ 6,330 | 现货 |
产品描述 | Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 . |
体外活性 | The AMC moiety is highest at 2 h with an area?of 5.6±1.8, the AMC peak area for Ac-DEVD-AMC incubated without a lung specimen (substrate alone) for 6 h is relatively small [1]. Ac-DEVD-AMC can be used to monitor intracellular caspase-3 activity[2]. |
细胞实验 | Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2]. |
别名 | AC-ASP-MET-GLN-ASP-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN |
分子量 | 675.64 |
分子式 | C30H37N5O13 |
CAS No. | 169332-61-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: ≤ 10 mg/mL (14.80 mM)
H2O: < 0.1 mg/mL (insoluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.4801 mL | 7.4004 mL | 14.8008 mL | 37.002 mL |
5 mM | 0.296 mL | 1.4801 mL | 2.9602 mL | 7.4004 mL | |
10 mM | 0.148 mL | 0.74 mL | 1.4801 mL | 3.7002 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Ac-DEVD-AMC 169332-61-0 Apoptosis Proteases/Proteasome Caspase Ac DEVD AMC AcDEVDAMC Inhibitor inhibit AC-ASP-MET-GLN-ASP-AMC AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN inhibitor