Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BF738735 是有效的磷脂酰肌醇 4-激酶 IIIβ 抑制剂,IC50为 5.7 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 346 | 现货 | ||
2 mg | ¥ 523 | 现货 | ||
5 mg | ¥ 990 | 现货 | ||
10 mg | ¥ 1,730 | 现货 | ||
25 mg | ¥ 3,890 | 现货 | ||
50 mg | ¥ 5,570 | 6-8周 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,090 | 现货 |
产品描述 | BF738735 is a selective inhibitor of phosphatidylinositol 4-kinase III beta (PI4KIIIβ, IC50 = 5.7 nM) showing higher activity over α(IC50 = 1.7 μM). BF738735 exhibits a broad spectrum of antiviral activity. |
靶点活性 | PI4KIIIα:1.7 μM, PI4KIIIβ:5.7 nM |
体外活性 | BF738735 effectively inhibits all viruses tested, with EC50s ranging between 4 and 71 nM. The cytotoxicity of BF738735, determined in parallel with the EC50 and using the same culture conditions for 3 to 4 days, is low, with CC50 values ranging from 11 to 65 μM, resulting in high selectivity indices. BF738735 exhibits a broad spectrum of antiviral activity, as it inhibits all tested species of enteroviruses and rhinoviruses, with 50% effective concentrations ranging between 4 and 71 nM. BF738735 also impairs PI4KIIIα, but only at an ~300-fold-higher concentration (IC50 of 1.7 μM). The activity of BF738735 is analyzed on a set of 150 cellular kinases, including 13 lipid kinases at a concentration of 10 μM. For all kinases, the inhibition is less than 10%, indicating that BF738735 specifically inhibits PI4KIIIβ in vitro. Low concentrations of BF738735 decrease the amount of luciferase to GuaHCl-treated levels, suggesting that the BF738735 blocks viral RNA replication. The EC50 of BF738735 in this assay is 77 nM, which is comparable to the inhibition observed in the multicycle assay for coxsackievirus serotype B3 (CVB3)[1]. |
体内活性 | BF738735 is well tolerated by specimens with good plasma levels of the antiviral in circulation. A complete inhibition with 25 mg/kg and some inhibition with 5 mg/kg dose is observed[2]. |
分子量 | 426.46 |
分子式 | C21H19FN4O3S |
CAS No. | 1436383-95-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 125 mg/mL (293.11 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.3449 mL | 11.7244 mL | 23.4489 mL | 58.6221 mL |
5 mM | 0.469 mL | 2.3449 mL | 4.6898 mL | 11.7244 mL | |
10 mM | 0.2345 mL | 1.1724 mL | 2.3449 mL | 5.8622 mL | |
20 mM | 0.1172 mL | 0.5862 mL | 1.1724 mL | 2.9311 mL | |
50 mM | 0.0469 mL | 0.2345 mL | 0.469 mL | 1.1724 mL | |
100 mM | 0.0234 mL | 0.1172 mL | 0.2345 mL | 0.5862 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
BF738735 1436383-95-7 Immunology/Inflammation PI3K/Akt/mTOR signaling Antiviral PI4K Phosphatidylinositol 4 kinases Inhibitor PI4 kinases BF-738735 inhibit Reverse Transcriptase BF 738735 inhibitor