Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NS-2028 是一种高度选择性的可溶性鸟苷酸环化酶抑制剂。它抑制 3-吗啉代-sydnonimine 在人培养的脐静脉内皮细胞中形成环状 GMP,IC50为30mM。它抑制小鼠小脑匀浆和神经元 NO 合酶中的可溶性鸟苷酸环化酶活性,IC50分别为 17 和 20 mM。它可降低血管内皮生长因子诱导的血管生成和通透性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 582 | 现货 | ||
2 mg | ¥ 848 | 现货 | ||
5 mg | ¥ 1,230 | 现货 | ||
10 mg | ¥ 2,180 | 现货 | ||
25 mg | ¥ 3,680 | 现货 | ||
50 mg | ¥ 5,330 | 现货 | ||
100 mg | ¥ 7,490 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,320 | 现货 |
产品描述 | NS-2028 is a highly selective soluble Guanylyl Cyclase (sGC) inhibitor (IC50: 30 nM and 200 nM for basal and NO-stimulated enzyme activity). It is commonly used in the research of nitric oxide signaling pathways. NS-2028 inhibits soluble Guanylyl Cyclase activity in homogenates of mouse cerebellum and neuronal NO synthase (IC50: 17 nM and 20 nM). NS-2028 inhibits 3-morpholino-sydnonimine (SIN-1)-elicited formation of cyclic GMP in human cultured umbilical vein endothelial cells (IC50: 30 nM). NS-2028 inhibits NO-dependent relaxant responses in non-vascular smooth muscle completely (1 μM). NS-2028 decreases vascular endothelial growth factor-induced angiogenesis and permeability. |
靶点活性 | sGC:30 nM |
体外活性 | NS-2028 (10 μM; 30 mins) decreases VEGF-induced EC migration by inhibiting p38 MAPK activation. NS-2028 (10 μM; 24 hours) inhibits 25% cell number in comparation with those grown in the presence of a vehicle[2]. |
体内活性 | In the avascular rabbit cornea, NS-2028 (p.o; 1 g/L; 8 days) displays an obvious reduction of new vessel formation in response to VEGF pellet implants[2]. |
分子量 | 269.05 |
分子式 | C9H5BrN2O3 |
CAS No. | 204326-43-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 250 mg/mL (929.20 mM), sonification is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.7168 mL | 18.5839 mL | 37.1678 mL | 92.9195 mL |
5 mM | 0.7434 mL | 3.7168 mL | 7.4336 mL | 18.5839 mL | |
10 mM | 0.3717 mL | 1.8584 mL | 3.7168 mL | 9.292 mL | |
20 mM | 0.1858 mL | 0.9292 mL | 1.8584 mL | 4.646 mL | |
50 mM | 0.0743 mL | 0.3717 mL | 0.7434 mL | 1.8584 mL | |
100 mM | 0.0372 mL | 0.1858 mL | 0.3717 mL | 0.9292 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NS-2028 204326-43-2 GPCR/G Protein Others Guanylate cyclase inhibit Inhibitor NS2028 Guanylate Cyclase NS 2028 inhibitor