Powder: -20°C for 3 years | In solvent: -80°C for 1 year
hPGDS-IN-1是 hPGDS 抑制剂,使用荧光偏振检测或通过 EIA 法测得的 IC50值为12 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 913 | 现货 | ||
5 mg | ¥ 2,450 | 现货 | ||
10 mg | ¥ 3,650 | 现货 | ||
25 mg | ¥ 5,880 | 现货 | ||
50 mg | ¥ 8,180 | 现货 | ||
100 mg | ¥ 10,800 | 现货 | ||
500 mg | ¥ 21,800 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 2,690 | 现货 |
产品描述 | hPGDS-IN-1 is a hPGDS inhibitor. |
靶点活性 | HPGDS:12 nM |
激酶实验 | TR-FRET biochemical assay with MET wild type and mutants: Enzyme activity is measured in a time resolved fluorescence resonance energy transfer (TR-FRET) assay, detecting tyrosine phosphorylation with a Eu-labelled anti-phospho-tyrosine antibody (fluorescence donor) and Allophycocyanin conjugated to Streptavidin (fluorescence acceptor) which binds to a biotin on the substrate peptide. For each variant, Km concentrations for ATP are determined in the absence of NVP-BVU972, and the ATP concentration in the kinase reaction is set to Km (4 μM for MET wt, 1 μM for MET Y1230H and MET F1200I). NVP-BVU972 is dissolved and diluted in DMSO and assayed in quadruplicate. Kinase reactions are carried out in 50 mM Tris-HCl pH 7.5, 8 mM MgCl2, 4 mM MnCl2, 0.05 % Tween 20, 0.05% bovine serum albumin, 0.1 mM EDTA, 1 mM DTT, 0.1 mM Na3VO4, in white 1536 well plates at room temperature. NVP-BVU972 and enzyme are incubated in a volume of 2 μL for 20 min, followed by the addition of 1 μL ATP and 1 μL biotinylated peptide substrate (PTK1) to final concentrations of Km and 1 μM, respectively. Enzyme concentrations in the reactions are 5 nM for MET wt, and 4 nM for the F1200I and Y1230H variants. After 90 min, reactions are stopped by addition of 1 μL stop/detection solution to reach final concentrations of 10 mM EDTA, 3.5 nM Eu-labelled antiphospho-tyrosine antibody PY20, and 10 nM Streptavidin Allophycocyanin. Time resolved fluorescence resonance energy transfer is measured in an Envision plate reader (excitation 320 nm, emission 615 nm and 665 nm). |
分子量 | 416.43 |
分子式 | C22H20N6O3 |
CAS No. | 1234708-04-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 30 mg/mL (72.04 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.4014 mL | 12.0068 mL | 24.0136 mL | 60.0341 mL |
5 mM | 0.4803 mL | 2.4014 mL | 4.8027 mL | 12.0068 mL | |
10 mM | 0.2401 mL | 1.2007 mL | 2.4014 mL | 6.0034 mL | |
20 mM | 0.1201 mL | 0.6003 mL | 1.2007 mL | 3.0017 mL | |
50 mM | 0.048 mL | 0.2401 mL | 0.4803 mL | 1.2007 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
hPGDS-IN-1 1234708-04-3 Immunology/Inflammation PGE Synthase Inhibitor inhibit hPGDS IN 1 Prostaglandin E synthase hPGDSIN1 PGE synthase inhibitor