store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Chaetocin 特异性抑制组蛋白甲基转移酶(HMT) SU(VAR)3-9,IC50值为0.6 μM。它还可抑制硫氧还蛋白还原酶,IC50值为4 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 1,130 | 现货 | ||
5 mg | ¥ 3,730 | 现货 | ||
10 mg | ¥ 5,370 | 现货 | ||
25 mg | ¥ 8,320 | 现货 | ||
50 mg | ¥ 11,200 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 4,680 | 现货 |
产品描述 | Chaetocin, a natural product from Chaetomium species, is a histone methyltransferase inhibitor with IC50 of 0.8 μM, 2.5 μM and 3 μM for dSU(VAR)3-9, mouse G9a and Neurospora crassa DIM5, respectively. |
靶点活性 | DIM5 (neurospora crassa):3 μM, G9a (mouse):2.5 μM, dSU(VAR)3-9:0.8 μM |
体外活性 | In SL-2 Drosophila tissue culture cells, Chaetocin causes the inhibition of SU(VAR)3-9 and the number of H3 molecules dimethylated at Lys9, and also inhibits cell growth. [1] In HepG2, Hep3B, and Huh7 human hepatoma cells, Chaetocin inhibits HIF-1-Mediated hypoxic responses. [2] Chaetocin also potently inhibits proliferation and colony formation in a broad range of cancer cell lines with IC50 of 2-10 nM. [3] |
体内活性 | In Hepa 1c1c-7 tumor-bearing mice, Chaetocin (0.25 mg/kg, i.p.) inhibits tumor growth by deregulating HIF-1[alpha]-mediated angiogenesis. [2] In SKOV3 tumor-bearing nude mice, chaetocin treatment (0.25 mg/kg, i.p.) significantly delays the tumor growth with minimal evidence of toxicities. [3] |
细胞实验 | HeLa cells are transfected with 1 μg pcDNA or pcDNA-Trx. Twenty four h after transfection the cells are treated with either DMSO, 100 nM chaetocin or 100 nM doxorubicin for 24 h. The cells are then trypsinized and manually counted in trypan blue to exclude dead cells. For immunoblotting (24 h after transfections), cells are trypsinized, ished in cold PBS, and lysed in CelLytic lysis buffer containing protease inhibitors. Protein is analyzed by BCA assay and lysates are electrophoresed on 15% SDS-PAGE gels and transferred to nitrocellulose. Immunoblotting for thioredoxin and actin is then performed[2]. |
别名 | 毛壳素 |
分子量 | 696.84 |
分子式 | C30H28N6O6S4 |
CAS No. | 28097-03-2 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 7 mg/mL (10 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.435 mL | 7.1752 mL | 14.3505 mL | 35.8762 mL |
5 mM | 0.287 mL | 1.435 mL | 2.8701 mL | 7.1752 mL | |
10 mM | 0.1435 mL | 0.7175 mL | 1.435 mL | 3.5876 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Chaetocin 28097-03-2 Chromatin/Epigenetic Microbiology/Virology Antibiotic Antibacterial Histone Methyltransferase Inhibitor inhibit 毛壳素 Bacterial inhibitor