store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Thrombin (MW 37kDa) 是一种 Na+激活的胰蛋白酶样别构丝氨酸蛋白酶。它能够利用凝血酶识别序列对融合蛋白进行位点特异性切割,并可用于消化 GST 标记的蛋白。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1000 U | ¥ 1,390 | 5日内发货 | ||
2000 U | ¥ 2,320 | 5日内发货 |
产品描述 | Thrombin (MW 37kDa) is a trypsin-like allosteric serine protease. |
体外活性 | Low concentrations of thrombin and thrombin preconditioning yield the potential of rescuing cells and to induce survival of neurons and astrocytes exposed to various ischemic insults[1]. |
体内活性 | Elevated thrombin activity was found in the brain slices from mice that were treated (in vitro and in vivo) with paraoxon. Increased levels of PAR-1 and pERK proteins and decreased prothrombin mRNA were found in the brains of paraoxon-treated mice. Furthermore, ex vivo and in vivo electrophysiological experiments showed that exposure to paraoxon causes elevated electrical activity in CA1 and CA3 regions of the hippocampus. Moreover, a specific PAR-1 antagonist (SCH79797) reduced this activity. Altogether, these results reveal the importance of thrombin and PAR-1 in paraoxon poisoning[2]. |
别名 | 凝血酶, Thrombin |
分子量 | 37kDa |
CAS No. | 9002-04-4 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Thrombin (MW 37kDa) 9002-04-4 Proteases/Proteasome Thrombin Inhibitor 凝血酶 inhibit inhibitor