EGFR Protein, Human, Recombinant (aa 1-645, His & Avi), Biotinylated

产品编号 TMPY-05612 EGFR Protein, Human, Recombinant (aa 1-645, His & Avi), Biotinylated

EGFR Protein, Human, Recombinant (aa 1-645, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 71.9 kDa and the accession number is P00533-1.

EGFR Protein, Human, Recombinant (aa 1-645, His & Avi), Biotinylated

产品编号 TMPY-05612 EGFR Protein, Human, Recombinant (aa 1-645, His & Avi), Biotinylated

规格	价格	库存	数量
20 μg	¥ 2,940	5日内发货
100 μg	¥ 6,800	5日内发货

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产品信息

生物活性	1. Immobilized EGF Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated, the EC50 is 280-900 ng/mL. 2. Immobilized Anti-EGFR(MK)-IgG1 Antibody (Cetuximab) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated, the EC50 is 5-30 ng/mL.
产品描述	EGFR Protein, Human, Recombinant (aa 1-645, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 71.9 kDa and the accession number is P00533-1.
种属	Human
表达系统	HEK293 Cells
标签	His, Avi
蛋白编号	P00533-1
别名	PIG61,NISBD2,mENA,HER1,ERBB1,ERBB,epidermal growth factor receptor
蛋白构建	A DNA sequence encoding the human EGFR (NP_005219.2) (Met1-Ser645) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Leu 25
蛋白纯度	> 95 % as determined by SDS-PAGE. >95% as determined by SEC-HPLC
分子量	71.9 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy