Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ZD7288 (ICI D7288) 是一种选择性超极化激活的环核苷酸门控通道阻滞剂,可抑制海马突触可塑性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 497 | 现货 | ||
2 mg | ¥ 738 | 现货 | ||
5 mg | ¥ 1,180 | 现货 | ||
10 mg | ¥ 2,120 | 现货 | ||
25 mg | ¥ 3,630 | 现货 | ||
50 mg | ¥ 5,230 | 现货 | ||
100 mg | ¥ 7,380 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,320 | 现货 |
产品描述 | ZD7288 (ICI D7288), a selective hyperpolarization-activated cyclic nucleotide-gated channel blocker, inhibits hippocampal synaptic plasticity. |
体外活性 | ZD7288 attenuated glutamate-induced rises in [Ca(2+)]i in a concentration-dependent manner and reversed 8-Br-cAMP-mediated facilitation of these glutamate-induced [Ca(2+)]i rises.?ZD7288 inhibits hippocampal synaptic plasticity both glutamate release and resultant [Ca(2+)]i increases in rat hippocampal neurons[1]. |
细胞实验 | Cultured hippocampal neurons were incubated with 1 μM Fura-2 acetoxy-methylol ester for 30 minutes at 37°C, washed three times with artificial cerebrospinal fluid (containing 140 mM NaCl, 5 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 10 mM glucose and 10 mM hydroxyethyl piperazine ethanesulfonic acid, pH 7.3), and then incubated at room temperature in the dark for 30 minutes.?Fura-2 fluorescence was observed by a Ratio Vision digital fluorescence microscopy system.?Fluorescence signals were evoked by 340 and 380 nm excitation wavelengths and collected at 510 nm by TILLvisION 4.0 software.?The 340:380 nm fluorescence ratio was used to represent [Ca2+]i.?Peak calcium change was represented as the percentage increase from baseline.?Neurons were incubated in ZD7288 (25, 50 or 100 μM) or 8-Br-cAMP (5 or 50 μM) for 15 minutes prior to stimulation with 50 μM glutamate.?All experiments were repeated in triplicate, using different batches of cells across 4–5 dishes[1]. |
别名 | N-乙基-1,6-二氢-1,2-二甲基-6-(甲基亚氨基)-N-苯基-4-嘧啶胺盐酸盐, ICI D7288 |
分子量 | 292.81 |
分子式 | C15H21ClN4 |
CAS No. | 133059-99-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (170.76 mM)
H2O: 50 mg/mL (170.76 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 3.4152 mL | 17.0759 mL | 34.1518 mL | 85.3796 mL |
5 mM | 0.683 mL | 3.4152 mL | 6.8304 mL | 17.0759 mL | |
10 mM | 0.3415 mL | 1.7076 mL | 3.4152 mL | 8.538 mL | |
20 mM | 0.1708 mL | 0.8538 mL | 1.7076 mL | 4.269 mL | |
50 mM | 0.0683 mL | 0.3415 mL | 0.683 mL | 1.7076 mL | |
100 mM | 0.0342 mL | 0.1708 mL | 0.3415 mL | 0.8538 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ZD7288 133059-99-1 Membrane transporter/Ion channel HCN Channel inhibit N-乙基-1,6-二氢-1,2-二甲基-6-(甲基亚氨基)-N-苯基-4-嘧啶胺盐酸盐 Hyperpolarization activated cyclic nucleotide gated channels ICI D-7288 ICI D7288 ICI D 7288 ZD-7288 ZD 7288 Inhibitor inhibitor