Powder: -20°C for 3 years | In solvent: -80°C for 1 year
L-Ascorbic acid 2-phosphate magnesium (2-Phospho-L-ascorbic acid magnesium) 是长效的维生素 C 衍生物,能够以刺激胶原蛋白的表达和形成。它提高成骨细胞分化过程中 hASC 中的碱性磷酸酶特性和 runx2A 的表达。它能够以作为培养基补充人脂肪干细胞的成骨分化。
产品描述 | L-Ascorbic acid 2-phosphate magnesium (2-Phospho-L-ascorbic acid magnesium) acts as an antioxidant and a stimulator of hepatocyte growth factor (HGF) production.It is a long-acting vitamin C derivative that can stimulate collagen formation and expression. |
体外活性 | L-Ascorbic acid 2-phosphate (50 μM-250 μM) is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 μM AsA2-P and 10 nM dexamethasone (Dex), and 250 μM AsA2-P and 5 nM Dex.L-Ascorbic acid 2-phosphate (0.1-1.5 mM;?2 to 3 weeks with medium exchange every 2 to 3 days) significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation.?A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect. |
别名 | 2-Phospho-L-ascorbic acid magnesium, 维生素C磷酸酯镁, 维生素 C 磷酸酯镁 |
分子量 | 292.56 |
分子式 | C6H9O9P.3/2Mg |
CAS No. | 113170-55-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: Slightly soluble
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
L-Ascorbic acid 2-phosphate magnesium 113170-55-1 Metabolism Phosphatase Phosphorylase Reactive Oxygen Species medium vitamin Endogenous Metabolite osteogenic 2-Phospho-L-ascorbic acid magnesium L Ascorbic acid 2 phosphate magnesium supplement runx2A derivative 维生素C磷酸酯镁 LAscorbic acid 2phosphate magnesium Inhibitor hASC differentiation 维生素 C 磷酸酯镁 inhibit inhibitor