Powder: -20°C for 3 years | In solvent: -80°C for 1 year
6-Methylcoumarin (Toncarine) 是一种合成香料,广泛用于化妆品中。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 129 | 现货 | ||
1 g | ¥ 163 | 现货 | ||
5 g | ¥ 289 | 现货 | ||
10 g | ¥ 413 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 99 | 现货 |
产品描述 | 6-Methylcoumarin (Toncarine) (6MC) is a semisynthetic coumarin with important in vitro and in vivo anti-inflammatory activity. |
体外活性 | Transport experiments with Caco-2 cells showed that 6MC presented high permeability at all concentrations evaluated. suggested that 6MC could be transported across the gut wall by passive diffusion |
体内活性 | 6-Methylcoumarin(6MC) could be transported across the gut wall by passive diffusion. The maximum concentration (Cmax) was 17.13 ± 2.90 μg/mL at maximum time (Tmax) of 30 min for the oral route and Cmax 26.18 ± 2.47 μg/mL at 6.0 min for the intraperitoneal administration, with elimination constant of (Ke ) 0.0070 min-1 and a short life half time of (T1/2 ) lower that 120 min. 6MC has high accumulation in the liver, and widespread distribution in all the organs evaluated. |
细胞实验 | The determination of in vitro intestinal permeability of 6MC was carried out under sink conditions in a series of pH-gradient bidirectional transport experiments with Caco-2 cells. Before the experiments, cell monolayers were rinsed with Hank's balanced salt solution (HBSS) and equilibrated for 30 min at 37 °C. The integrity of the monolayers was assessed before and after the experiments, by transepithelial electrical resistance (TEER) measurement. Only monolayers with TEER values above 200 Ωcm2 were considered. A stock solution of 6MC (10 mM DMSO) was diluted to final concentration of 10, 25, 50 or 100 μM in HBSS pH 6.5 (apical transport buffer) or pH 7.4 (basolateral transport buffer). Bidirectional experiments (apical-to-basolateral [AB] and basolateral-to-apical [BA]) were initiated by adding 6MC solutions to the donor compartment, and fresh buffer to the acceptor compartment. Caco-2 cell monolayers were incubated for 1 h at 37 °C under constant stirring (150 rpm). Receiver compartments were sampled at 0, 15, 30, 45 and 60 min, refilled with an equivalent amount of transport buffer, and samples were submitted to analysis by HPLC/UV. |
别名 | Toncarine, 6-甲基香豆素 |
分子量 | 160.17 |
分子式 | C10H8O2 |
CAS No. | 92-48-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/mL (343.39 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 6.2434 mL | 31.2168 mL | 62.4337 mL | 156.0842 mL |
5 mM | 1.2487 mL | 6.2434 mL | 12.4867 mL | 31.2168 mL | |
10 mM | 0.6243 mL | 3.1217 mL | 6.2434 mL | 15.6084 mL | |
20 mM | 0.3122 mL | 1.5608 mL | 3.1217 mL | 7.8042 mL | |
50 mM | 0.1249 mL | 0.6243 mL | 1.2487 mL | 3.1217 mL | |
100 mM | 0.0624 mL | 0.3122 mL | 0.6243 mL | 1.5608 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
6-Methylcoumarin 92-48-8 Others inhibit Toncarine Inhibitor 6 Methylcoumarin 6Methylcoumarin 6-甲基香豆素 inhibitor