Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Alendronate sodium hydrate (MK 217) 是 farnesyl diphosphate synthase 抑制剂(IC50:460 nM)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 148 | 现货 | ||
50 mg | ¥ 220 | 现货 | ||
100 mg | ¥ 417 | 现货 | ||
200 mg | ¥ 648 | 现货 |
产品描述 | Alendronate sodium hydrate (MK 217) is a nitrogen-containing bisphosphonate, which is an effective inhibitor of bone resorption. It is utilized for the treatment and prevention of osteoporosis. |
靶点活性 | FPPS:460 nM. |
体外活性 | 在小鼠中,一周一至两次0.1 mg/kg Alendronate预处理并联合使用10-50 mg/kg紫杉醇给药,可以阻断骨髓和软组织中PC-3 ML肿瘤的生长,并明显将生存时间提高到4-5周.在大鼠体内,Alendronate会增强吲哚美辛诱发的胃损伤,并延缓胃溃疡恢复.在兔子体内,Alendronate引起胃糜烂,同时增加吲哚美辛诱发的胃窦溃疡的发病率和面积. |
体内活性 | 阿仑膦酸钠,主要在破骨细胞中抑制胆固醇生物合成途径中的限速步骤。阿仑膦酸钠通过降低的香叶基香叶基二磷酸酯的水平,它可以干扰蛋白质异戊烯化,同时抑制类异戊二烯的生物合成途径。阿仑膦酸钠还以抑制0破骨细胞中的甾醇。 |
激酶实验 | Rat liver cytosol is prepared from a separate piece of liver from rat. Assays are carried out in a total volume of 0.1 mL containing 10 mg of cytosolic protein, and components according to Rilling. All reaction components (except IPP) are mixed and kept on ice for 15 min. Reactions are initiated by the addition of [14C]IPP and incubation at 37°C. Reactions are stopped after 5 min by addition of 0.4 mL MeOH/HCl (4/1, by vol.) and the samples are incubated a further 15 min to hydrolyze the allylic pyrophosphates to petroleum ether-extractable products. Following addition of 0.5 mL water and 1 mL petroleum ether, 50% of the upper (petroleum ether-extractable) phase is taken for liquid scintillation analysis. Preliminary experiments indicated that the reaction is linear with time and protein under these conditions, and no more than 10% of the substrate is consumed. |
别名 | G-704650, Alendronate, MK 217, Alendronate sodium trihydrate, 阿仑膦酸钠, G-704650 Adronat, Fosamax |
分子量 | 325.12 |
分子式 | C4H12NO7P2·3H2O·Na |
CAS No. | 121268-17-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: Slightly soluble
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Alendronate sodium hydrate 121268-17-5 Metabolism Transferase MK-217 G-704650 Alendronate sodium Trihydrate Alendronate inhibit Alendronate sodium MK 217 Alendronate sodium trihydrate MK217 Inhibitor G 704650 阿仑膦酸钠 G-704650 Adronat Fosamax Alendronate sodium Hydrate G704650 inhibitor