Anti-HDAC3 Antibody (6T538) is an antibody targeting HDAC3. Anti-HDAC3 Antibody (6T538) can be used in ELISA, WB, IHC.

Rabbit IgG

6T538

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate, 293T whole cell lysate
-All lanes: HDAC3 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 49, 50 kDa
-Observed band size: 49 kDa
2. IHC image of TMAH-00536 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00536 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

WB:1:500-1:5000; IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human HDAC3

Human

Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism, Stem cells