Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SMI-16a (PIM1/2 Kinase Inhibitor VI) 是选择性 Pim 激酶抑制剂,对 Pim1、Pim2 和 PC3 细胞的 IC50值分别为0.15、0.02 和48 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 248 | 现货 | ||
2 mg | ¥ 368 | 现货 | ||
5 mg | ¥ 632 | 现货 | ||
10 mg | ¥ 877 | 现货 | ||
25 mg | ¥ 1,620 | 现货 | ||
50 mg | ¥ 2,450 | 现货 | ||
100 mg | ¥ 3,660 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 723 | 现货 |
产品描述 | SMI-16a (PIM1/2 Kinase Inhibitor VI) , a cell-permeable thiazolidinedione compound, acts as an effective, ATP-competitive inhibitor against Pim-1/2 kinases (IC50: 150/20 nM) while exhibiting little or no activity against a panel of 57 other kinases (≤18% inhibition at 5 μM). |
靶点活性 | Pim1:150 nM, Pim2:20 nM |
体外活性 | PIM1/2 Kinase Inhibitor VI exhibits antitumor activity in PC3 human prostate cancer cultures in vitro (IC50: 48 μM). |
体内活性 | PIM1/2 Kinase Inhibitor VI exhibits antitumor activity in JC adenocarcinoma-transplanted Balb/C mice in vivo (~46% tumor mass reduction on day 20; 50 mg/kg/day, i.p.). |
激酶实验 | Competition binding reactions used 25 μg human M1 CHO membrane protein, BQCA or vehicle, and 0.15 nM [3H]NMS in 96-well deep-well plates. Binding reactions (30 °C for 2-3 h) are terminated by rapid filtration. Nonspecific binding is determined by adding 10 μM atropine. Filter plates are ished 4×with ice-cold 20 mM HEPES, 100 mM NaCl, and 5 mM MgCl2, pH 7.4 using a 96-well harvester. Plates are dried and radioactivity counted with a microplate scintillation counter[1]. |
别名 | PIM1/2 Kinase Inhibitor VI |
分子量 | 263.31 |
分子式 | C13H13NO3S |
CAS No. | 587852-28-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 150 mg/mL (569.67 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.7978 mL | 18.989 mL | 37.978 mL | 94.9451 mL |
5 mM | 0.7596 mL | 3.7978 mL | 7.5956 mL | 18.989 mL | |
10 mM | 0.3798 mL | 1.8989 mL | 3.7978 mL | 9.4945 mL | |
20 mM | 0.1899 mL | 0.9495 mL | 1.8989 mL | 4.7473 mL | |
50 mM | 0.076 mL | 0.3798 mL | 0.7596 mL | 1.8989 mL | |
100 mM | 0.038 mL | 0.1899 mL | 0.3798 mL | 0.9495 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SMI-16a 587852-28-6 Chromatin/Epigenetic JAK/STAT signaling Pim SMI 16a inhibit SMI16a PIM1/2 Kinase Inhibitor VI Inhibitor Pim kinases inhibitor