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ANNINE-6plus

ANNINE-6plus

产品编号 T30078   CAS 1151942-85-6

ANNINE-6plus is a water soluble voltage sensitive dye (also called potentiometric dyes). This compound was developed at the Max Planck Institute for Biochemistry in Germany. It is used to optically measure the changes in transmembrane voltage of excitable cells, including neurons, skeletal and cardiac myocytes. ANNINE-6plus has a fractional fluorescent intensity change (ΔF/F per 100 mV change) of about 30% with single-photon excitation (~488 nm) and >50% with two-photon excitation (~1060 nm).

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ANNINE-6plus Chemical Structure
ANNINE-6plus, CAS 1151942-85-6
规格 价格/CNY 货期 数量
100 mg 待询 待询
500 mg 待询 待询
产品目录号及名称: ANNINE-6plus (T30078)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 ANNINE-6plus is a water soluble voltage sensitive dye (also called potentiometric dyes). This compound was developed at the Max Planck Institute for Biochemistry in Germany. It is used to optically measure the changes in transmembrane voltage of excitable cells, including neurons, skeletal and cardiac myocytes. ANNINE-6plus has a fractional fluorescent intensity change (ΔF/F per 100 mV change) of about 30% with single-photon excitation (~488 nm) and >50% with two-photon excitation (~1060 nm).
分子量 717.62
分子式 C39H47Br2N3
CAS No. 1151942-85-6

存储

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Ramamoorthy S, Wilson TM, Wu T, Nuttall AL. Non-uniform distribution of outer hair cell transmembrane potential induced by extracellular electric field. Biophys J. 2013 Dec 17;105(12):2666-75. doi: 10.1016/j.bpj.2013.11.021. PubMed PMID: 24359738; PubMed Central PMCID: PMC3882456. 2. Wegner LH, Frey W, Schönwälder S. A critical evaluation of whole cell patch clamp studies on electroporation using the voltage sensitive dye ANNINE-6. Bioelectrochemistry. 2013 Aug;92:42-6. doi: 10.1016/j.bioelechem.2013.03.002. Epub 2013 Apr 3. PubMed PMID: 23603150. 3. Pagès S, Côté D, De Koninck P. Optophysiological approach to resolve neuronal action potentials with high spatial and temporal resolution in cultured neurons. Front Cell Neurosci. 2011 Oct 10;5:20. doi: 10.3389/fncel.2011.00020. eCollection 2011. PubMed PMID: 22016723; PubMed Central PMCID: PMC3191737. 4. Vignali S, Peter N, Ceyhan G, Demir IE, Zeller F, Senseman D, Michel K, Schemann M. Recordings from human myenteric neurons using voltage-sensitive dyes. J Neurosci Methods. 2010 Oct 15;192(2):240-8. doi: 10.1016/j.jneumeth.2010.07.038. Epub 2010 Aug 5. PubMed PMID: 20691728.

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

ANNINE-6plus 1151942-85-6 ANNINE 6plus ANNINE6plus Inhibitor inhibitor inhibit

 

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