Phosphatase Inhibitor Cocktail III (2 Tubes, 100x)

Catalog No. C0004

背景：在稳定的环境条件下细胞内蛋白质的生成和分解处于动态平衡，当在体外从细胞和组织中提取蛋白时，细胞内的蛋白酶和磷酸酶就会被释放出来或被激活，导致目的蛋白分解或去修饰。因此在提取物中添加适当的蛋白酶、磷酸酶等抑制剂是防止蛋白降解和去修饰的有效方法。

TargetMol 磷酸酶抑制剂 Cocktail III 是含有8种广谱磷酸酶抑制剂的混合物，高效抑制蛋白提取物中的酸性、碱性、丝氨酸/苏氨酸和酪氨酸磷酸酶。与同类产品（一般含3~4种抑制剂）相比，TargetMol 磷酸酶抑制剂 Cocktail III 可提供更全面的磷酸酶抑制能力。

TargetMol的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

Phosphatase Inhibitor Cocktail III (2 Tubes, 100x)

规格	价格/CNY	货期
1 mL I + 1 mL II	￥ 360	现货
(1 mL I + 1 mL II) * 10	￥ 2,640	现货
(1 mL I + 1 mL II) * 50	￥ 10,360	现货
(1 mL I + 1 mL II) * 100	￥ 15,200	现货

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产品组成

管号	Ingredient	CAS	MW	Concentration (100x)	Target	Type
I (ddH2O) 1mL	Sodium Fluoride	7681-49-4	41.99	100 mM	Acid Phosphatase	Reversible
	Sodium Orthovanadate	13721-39-6	183.91	100 mM	Alkaline phosphatase, PTPs, ATPases	Reversible
	Sodium Molybdate	7631-95-0	205.92	115 mM	Acid and phosphoprotein Phosphatase	Irreversible
	Sodium Tartrate	868-18-8	194.05	400mM	Acid Phosphatase	Reversible
	Imidazole	288-32-4	68.08	200 mM	Alkaline phosphatase	Reversible
II (DMSO) 1mL	(-)-p-Bromotetramisole oxalate	62284-79-1	373.22	2.5 mM	Alkaline phosphatases	Irreversible
	Cantharidin	56-25-7	196.2	500 μM	Ser/Thr phosphatases	Reversible
	Microcystin LR	101043-37-2	995.17	500 nM	Acid and PP1 and PP2A	Reversible

产品应用

在室温下解冻（如果DMSO在室温下融化较慢，可考虑25℃水浴融化），开盖前请低速离心一下，以便将黏附于管壁的液体甩至管底。然后在实验前以1:100（v / v）稀释至溶液样品（如细胞裂解液或组织提取液），短暂涡旋震荡加速溶解。应用于：WB、IP、Co-IP、pull-down、IF、IHC、kinase assay等。

包装与规格

Attribute	Value
Form	Liquid
Formulation	2 Tube Solution (100×)
Storage	-20 ℃ for one year, Avoid free/thaw
Freeze	Ok to freeze
Toxicity	Irritant

产品优势

组分清晰，浓度明确；
产品兼容性好，高效抑制多种磷酸酶，全面保护蛋白的磷酸化状态；

注意事项

注意如果将磷酸酶抑制剂Cocktail I 与Cocktail II一起使用，要先加Cocktail I于样品液中混匀，然后再加Cocktail II，再次混匀，不可将Cocktail I 与Cocktail II预先混匀，可能会产生沉淀，因此要分步加入。

文件下载

C0004说明书-中文(1.25 MB)

文献引用

1. Shi S, Gu H, Xu J, et al.Glia maturation factor beta deficiency protects against diabetic osteoporosis by suppressing osteoclast hyperactivity.Experimental & Molecular Medicine.2023: 1-12. 2. Zeng Z, Li T, Liu X, et al.DNA dioxygenases TET2 deficiency promotes cigarette smoke induced chronic obstructive pulmonary disease by inducing ferroptosis of lung epithelial cell.Redox Biology.2023: 102916. 3. Wang F, Qi Q, Qin B, et al.Targeting VCP potentiates immune checkpoint therapy for colorectal cancer.Cell Reports.2023, 42(11). 4. Jiao J, Ruan L, Cheng C, et al.Paired protein kinases PRKCI-RIPK2 promote pancreatic cancer growth and metastasis via enhancing NF-κB/JNK/ERK phosphorylation.Molecular Medicine.2023, 29(1): 1-17. 5. Meng Q, Wu W, Zhang W, et al.IL-18BP Improves Early Graft Function and Survival in Lewis–Brown Norway Rat Orthotopic Liver Transplantation Model.Biomolecules.2022, 12(12): 1801. 6. Zhang Y, Wu K, Liu Y, et al.UHRF2 promotes the malignancy of hepatocellular carcinoma by PARP1 mediated autophagy.Cellular Signalling.2023: 110782. 7. Liang J, Zhao G, Bian Y, et al.HNF4G increases cisplatin resistance in lung adenocarcinoma via the MAPK6/Akt pathway.PeerJ.2023, 11: e14996. 8. Jin J, Ou Q, Wang Z, et al. BMSC-derived extracellular vesicles intervened the pathogenic changes of scleroderma in mice through miRNAs. Stem cell research & therapy. 2021, 12(1): 1-16. 9. Yang H, Ting X, Geng Y H, et al. The risk variant rs11836367 contributes to breast cancer onset and metastasis by attenuating Wnt signaling via regulating NTN4 expression. Science Advances. 2022, 8(23): eabn3509 10. Li G N, Zhao X J, Wang Z, et al. Elaiophylin triggers paraptosis and preferentially kills ovarian cancer drug-resistant cells by inducing MAPK hyperactivation. Signal Transduction and Targeted Therapy. 2022, 7(1): 1-15.

11. Su J, Thakur A, Pan G, et al.Morus alba derived Kuwanon: A combined with 5‐fluorouracil reduce tumor progression via synergistic activation of GADD153 in gastric cancer.MedComm–Oncology.2023, 2(1): e24. 12. Hu H, Xu L, Luo S J, et al. Retinal dehydrogenase 5 (RHD5) attenuates metastasis via regulating HIPPO/YAP signaling pathway in Hepatocellular Carcinoma. International Journal of Medical Sciences. 2020, 17(13): 1897 13. Hu H, Luo S J, Cao Z, et al. Depressive Disorder promotes Hepatocellular Carcinoma metastasis via upregulation of ABCG2 gene expression and maintenance of self-renewal. Journal of Cancer. 2020, 11(18): 5309-5317 14. Zhong L, Ren X, Ai Y, et al. SS-31 Improves Cognitive Function in Sepsis-Associated Encephalopathy by Inhibiting the Drp1-NLRP3 Inflammasome Activation. NeuroMolecular Medicine. 2022: 1-12. 15. Tang H, Guo J, Sang B, et al. PDGFRβ Modulates Aerobic Glycolysis in Osteosarcoma HOS Cells via the PI3K/AKT/mTOR/c-Myc Pathway. Biochemistry and Cell Biology. 2021 (ja). 16. Liu H, Mi Q, Ding X, et al. Discovery and characterization of novel potent BCR-ABL degraders by conjugating allosteric inhibitor. European Journal of Medicinal Chemistry. 2022: 114810 17. Xue D D, Wei C L, Zhou Y H, et al. TRIOL Inhibits Rapid Intracellular Acidification and Cerebral Ischemic Injury: The Role of Glutamate in Neuronal Metabolic Reprogramming. ACS Chemical Neuroscience. 2022 18. Du T, Zhang Z, Zhou J, et al. A Novel PARP Inhibitor YHP-836 For the Treatment of BRCA-Deficiency Cancers. Frontiers in pharmacology. 2022, 13. 19. Shi Y, Cui X, Jiang T, et al. The therapeutic effect of KSP inhibitors in preclinical models of cholangiocarcinoma. Cell Death & Disease. 2022, 13(9): 1-13. 20. Zhang F, Hu G, Chen X, et al. Excessive branched-chain amino acid accumulation restricts mesenchymal stem cell-based therapy efficacy in myocardial infarction. Signal Transduction and Targeted Therapy. 2022, 7(1): 1-18 21. Li L, Xu X, Lv K, et al. Asebogenin Suppresses Thrombus Formation via Inhibition of Syk Phosphorylation. British Journal of Pharmacology. 2022 22. Lu B, Zhu Z, Sheng L, et al. SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury. Oxidative medicine and cellular longevity. 2020 Oct 23;2020:2048210 23. Niu Z, Wang L, Qin X, et al. Macrophage derived miR-7219-3p-containing exosomes mediate fibroblast trans-differentiation by targeting SPRY1 in silicosis. Toxicology. 2022: 153310. 24. Wu F, Xie X, Li G, et al. AKG induces cell apoptosis by inducing reactive oxygen species‐mediated endoplasmic reticulum stress and by suppressing PI3K/AKT/mTOR‐mediated autophagy in renal cell carcinoma. Environmental Toxicology. 2022 25. Hu S, Wang Z, Jin C, et al. Human amniotic epithelial cell-derived extracellular vesicles provide an extracellular matrix-based microenvironment for corneal injury repair. Journal of Tissue Engineering. 2022, 13: 20417314221122123 26. Li H, Liu N N, Li J R, et al. Combined Use of Bicyclol and Berberine Alleviates Mouse Nonalcoholic Fatty Liver Disease. Frontiers in pharmacology. 2022, 13: 843872-843872. 27. Guo D, Yang X, Gao M, et al. Deficiency of Autism-Related Gene Dock4 Leads to Impaired Spatial Memory and Hippocampal Function in Mice at Late Middle Age. Cellular and Molecular Neurobiology. 2022: 1-18 28. Li H, Liu N N, Li J R, et al. Bicyclol ameliorates advanced liver diseases in murine models via inhibiting the IL-6/STAT3 signaling pathway. Biomedicine & Pharmacotherapy. 2022, 150: 113083 29. Xiong T C, Wei M C, Li F X, et al. The E3 ubiquitin ligase ARIH1 promotes antiviral immunity and autoimmunity by inducing mono-ISGylation and oligomerization of cGAS. Nature Communications. 2022, 13(1): 1-19. 30. Li H, Liu N N, Li J R, et al. Combined Use of Bicyclol and Berberine Alleviates Mouse Nonalcoholic Fatty Liver Disease. Frontiers in pharmacology. 2022, 13: 843872-843872. 31. Qian H, Zhou T, Fu Y, et al. Self-assembled Tetrahedral Framework Nucleic Acid Mediated Tumor-associated Macrophages Reprogramming and Restores Antitumor Immunity. Molecular Therapy-Nucleic Acids. 2022 32. Jin C, Ou Q, Chen J, et al. Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis. Stem Cell Research & Therapy. 2022, 13(1): 1-14 33. Song X, Qiao L, Chang J, et al. Dietary supplementation with selenium nanoparticles-enriched Lactobacillus casei ATCC 393 alleviates intestinal barrier dysfunction of mice exposed to deoxynivalenol by regulating endoplasmic reticulum stress and gut microbiota. Ecotoxicology and Environmental Safety. 2022, 248: 114276. 34. Chen L, Qian Z, Zheng Y, et al.Structural analysis of PTPN21 reveals a dominant-negative effect of the FERM domain on its phosphatase activity.Science Advances.2024, 10(9): eadi7404.

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