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顶部生物活性储存和溶解信息计算器

Anti-RPA1 Antibody (1L894)

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Anti-RPA1 Antibody (1L894)
产品编号 TMAC-03629
别名 Single-stranded DNA-binding protein, RPA70, RPA1, RP-A p70, Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1 (RF-A protein 1), REPA1

Anti-RPA1 Antibody (1L894) 是一种 Rabbit 抗体，靶向 RPA1。Anti-RPA1 Antibody (1L894) 可用于 WB,ICC,IF,FCM。

Anti-RPA1 Antibody (1L894)

Anti-RPA1 Antibody (1L894)

Rating icon 还可以
Anti-RPA1 Antibody (1L894)
产品编号 TMAC-03629 别名 Single-stranded DNA-binding protein, RPA70, RPA1, RP-A p70, Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1 (RF-A protein 1), REPA1

Anti-RPA1 Antibody (1L894) 是一种 Rabbit 抗体，靶向 RPA1。Anti-RPA1 Antibody (1L894) 可用于 WB,ICC,IF,FCM。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-RPA1 Antibody (1L894) is a Rabbit antibody targeting RPA1. Anti-RPA1 Antibody (1L894) can be used in WB,ICC,IF,FCM.
别名Single-stranded DNA-binding protein, RPA70, RPA1, RP-A p70, Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1 (RF-A protein 1), REPA1
Ig Type
IgG
克隆号
1L894
交叉反应
Human
验证活性
1. Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.
2. ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining RPA70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining RPA70 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of 293T cells with RPA70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC
IF
FCM
推荐剂量
WB: 1:500; ICC: 1:50-100; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner.
偶联与修饰
偶联
Unconjugated
抗原信息
Uniprot ID
P27694
化学信息
分子量Theoretical: 68 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

RPA p70RPA 70RPA 1RF-A protein1RF-A protein 1REPA 1

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