Anti-Podoplanin Antibody (7J57) is an antibody targeting Podoplanin. Anti-Podoplanin Antibody (7J57) can be used in ELISA, WB, IHC, FCM.

Mouse IgG2a

7J57

Human, Mouse

1. Western Blot
-Positive WB detected in: PC-3 whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate, HepG2 whole cell lysate
-All lanes: PDPN antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 17 kDa
-Observed band size: 17 kDa
2. IHC image of TMAH-00874 diluted at 1:300 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00874 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
4. Overlay Peak curve showing 293 cells stained with TMAH-00874 (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,026 events was performed.

WB:1:1000-1:5000; IHC:1:20-1:200; FCM:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human PDPN Protein

Human

Cardiovascular, Tags & Cell Markers