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别名 P90, IP90, CNX, calnexin
Anti-Calnexin Antibody (2Q119) 是一种 Rabbit 抗体,靶向 Calnexin。Anti-Calnexin Antibody (2Q119) 可用于 FCM,ICC/IF,IHC,WB。
Anti-Calnexin Antibody (2Q119) 是一种 Rabbit 抗体,靶向 Calnexin。Anti-Calnexin Antibody (2Q119) 可用于 FCM,ICC/IF,IHC,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
| 产品描述 | Anti-Calnexin Antibody (2Q119) is a Rabbit antibody targeting Calnexin. Anti-Calnexin Antibody (2Q119) can be used in FCM,ICC/IF,IHC,WB. |
| 别名 | P90, IP90, CNX, calnexin |
| Ig Type | IgG |
| 克隆号 | 2Q119 |
| 交叉反应 | Human,Rat |
| 验证活性 | 1. Western blot analysis of Calnexin on Hela cells lysates using anti-Calnexin antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Calnexin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calnexin antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin. 8. ICC staining Calnexin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining Calnexin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining Calnexin in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 11. Flow cytometric analysis of Hela cells with Calnexin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
| 应用 | FCMICC/IFIHCWB |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Calnexin and Calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding ??E-F hand?? motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 90 kDa. |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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