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Anti-Calnexin Antibody (2Q119)

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Anti-Calnexin Antibody (2Q119)
产品编号 TMAC-00528
别名 P90, IP90, CNX, calnexin

Anti-Calnexin Antibody (2Q119) 是一种 Rabbit 抗体,靶向 Calnexin。Anti-Calnexin Antibody (2Q119) 可用于 WB,ICC/IF,IHC,FCM。

Anti-Calnexin Antibody (2Q119)

Anti-Calnexin Antibody (2Q119)

Rating icon 还可以
Anti-Calnexin Antibody (2Q119)
产品编号 TMAC-00528 别名 P90, IP90, CNX, calnexin

Anti-Calnexin Antibody (2Q119) 是一种 Rabbit 抗体,靶向 Calnexin。Anti-Calnexin Antibody (2Q119) 可用于 WB,ICC/IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-Calnexin Antibody (2Q119) is a Rabbit antibody targeting Calnexin. Anti-Calnexin Antibody (2Q119) can be used in WB,ICC/IF,IHC,FCM.
别名P90, IP90, CNX, calnexin
Ig Type
IgG
交叉反应
Human,Rat
验证活性
1. Western blot analysis of Calnexin on Hela cells lysates using anti-Calnexin antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
7. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
8. ICC staining Calnexin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. ICC staining Calnexin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. ICC staining Calnexin in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
11. Flow cytometric analysis of Hela cells with Calnexin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
推荐剂量
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Calnexin and Calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding ??E-F hand?? motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
化学信息
分子量Theoretical: 90 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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