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Anti-ZO-1/TJP1 Polyclonal Antibody 2
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产品编号 TMAB-01999
Anti-ZO-1/TJP1 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 ZO-1/TJP1。Anti-ZO-1/TJP1 Polyclonal Antibody 2 可用于 WB,ICC/IF,FCM。
Anti-ZO-1/TJP1 Polyclonal Antibody 2
Anti-ZO-1/TJP1 Polyclonal Antibody 2
还可以产品编号 TMAB-01999
Anti-ZO-1/TJP1 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 ZO-1/TJP1。Anti-ZO-1/TJP1 Polyclonal Antibody 2 可用于 WB,ICC/IF,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-ZO-1/TJP1 Polyclonal Antibody 2 is a Rabbit antibody targeting ZO-1/TJP1. Anti-ZO-1/TJP1 Polyclonal Antibody 2 can be used in WB,ICC/IF,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. 25 μg total protein per Lane of various lysates probed with ZO-1 polyclonal antibody, unconjugated (TMAB-01999) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. The MCF-7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-ZO-1 antibody (TMAB-01999): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. 3. The MCF-7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-ZO-1 antibody (TMAB-01999): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] |
| 免疫原 | KLH conjugated synthetic peptide: human ZO-1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Plasma Membrane |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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