Anti-SOCS3 Polyclonal Antibody is a Rabbit antibody targeting SOCS3. Anti-SOCS3 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Kidney (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: LympHnode (Rat) Lysate at 40 μg
Primary: Anti-SOCS3 (TMAB-01748) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 26 kDa
2. Blank control: U937. Primary Antibody (green line): Rabbit Anti-SOCS3 antibody (TMAB-01748)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Tes

Polyclonal

KLH conjugated synthetic peptide: human SOCS3

Human

Response to hypoxia,STATs,IL6 Related,Hypoxia,Obesity,Cytoplasmic,Intracellular Molecules