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Anti-SEMA4D Antibody (6F523)
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产品编号 TMAH-01082
别名 Semaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164
Anti-SEMA4D Antibody (6F523) 是一种 Mouse 抗体，靶向 SEMA4D。Anti-SEMA4D Antibody (6F523) 可用于 ELISA, WB, FCM。
Anti-SEMA4D Antibody (6F523)
Anti-SEMA4D Antibody (6F523)
还可以产品编号 TMAH-01082 别名 Semaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164
Anti-SEMA4D Antibody (6F523) 是一种 Mouse 抗体，靶向 SEMA4D。Anti-SEMA4D Antibody (6F523) 可用于 ELISA, WB, FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SEMA4D Antibody (6F523) is a Mouse antibody targeting SEMA4D. Anti-SEMA4D Antibody (6F523) can be used in ELISA, WB, FCM. |
| 别名 | Semaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164 |
| Ig Type | IgG2b |
| 克隆号 | 6F523 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: 293T whole cell lysate transfected with SEMA4D; Recombinant proteins with SEMA4D -All lanes: SEMA4D antibody at 1:1000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 130, 150 KDa -Observed band size: 130, 150 KDa -Exposure time：5min 2. Overlay Peak curve showing 293T whole cell lysate transfected with SEMA4D stained with TMAH-01082 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. |
| 组织特异性 | Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| 纯度 | >95% |
| 研究背景 | Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human SEMA4D Protein(22-734aa) |
| 抗原种属 | Human |
| 基因名称 | SEMA4D |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Signal Transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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