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Anti-S100A8 Antibody (4J730)
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产品编号 TMAB-01644
Anti-S100A8 Antibody (4J730) 是一种 Mouse 抗体，靶向 S100A8。Anti-S100A8 Antibody (4J730) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-S100A8 Antibody (4J730)
Anti-S100A8 Antibody (4J730)
还可以产品编号 TMAB-01644
Anti-S100A8 Antibody (4J730) 是一种 Mouse 抗体，靶向 S100A8。Anti-S100A8 Antibody (4J730) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-S100A8 Antibody (4J730) is a Mouse antibody targeting S100A8. Anti-S100A8 Antibody (4J730) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 4J730 |
| 交叉反应 | Human (predicted:Mouse,Rat) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (TMAB-01644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (TMAB-01644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (TMAB-01644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Sample: Lane 1: Recombinant human S100-A8/A9 protein, His at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 34 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC-P: 1:100-200; IHC-Fr: 1:400-800; IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq]. |
| 免疫原 | Recombinant Protein: human human S100A8+A9 Complex |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Macrophage / Inflamm.,Calcium Binding Proteins |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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