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顶部生物活性储存和溶解信息计算器

Anti-RF1 Antibody (8B803)

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Anti-RF1 Antibody (8B803)
产品编号 TMAC-03589
别名 TB3-1, TB3 1, SUP45L1, Sup45 (yeast omnipotent suppressor 45) homolog like 1, RF1, RF 1, Protein Cl1, Polypeptide chain release factor 1, MGC111066, Eukaryotic translation termination factor 1, Eukaryotic release factor 1, Eukaryotic peptide chain release factor subunit 1, ETF1, ERF1_HUMAN, eRF1, ERF, D5S1995, Cl1 protein

Anti-RF1 Antibody (8B803) 是一种 Mouse 抗体，靶向 RF1。Anti-RF1 Antibody (8B803) 可用于 WB,FCM。

Anti-RF1 Antibody (8B803)

Anti-RF1 Antibody (8B803)

Rating icon 还可以
Anti-RF1 Antibody (8B803)
产品编号 TMAC-03589 别名 TB3-1, TB3 1, SUP45L1, Sup45 (yeast omnipotent suppressor 45) homolog like 1, RF1, RF 1, Protein Cl1, Polypeptide chain release factor 1, MGC111066, Eukaryotic translation termination factor 1, Eukaryotic release factor 1, Eukaryotic peptide chain release factor subunit 1, ETF1, ERF1_HUMAN, eRF1, ERF, D5S1995, Cl1 protein

Anti-RF1 Antibody (8B803) 是一种 Mouse 抗体，靶向 RF1。Anti-RF1 Antibody (8B803) 可用于 WB,FCM。

规格价格库存数量
50 μL
¥ 1,980
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100 μL
¥ 2,985
5日内发货
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产品介绍

生物活性
产品描述
Anti-RF1 Antibody (8B803) is a Mouse antibody targeting RF1. Anti-RF1 Antibody (8B803) can be used in WB,FCM.
别名TB3-1, TB3 1, SUP45L1, Sup45 (yeast omnipotent suppressor 45) homolog like 1, RF1, RF 1, Protein Cl1, Polypeptide chain release factor 1, MGC111066, Eukaryotic translation termination factor 1, Eukaryotic release factor 1, Eukaryotic peptide chain release factor subunit 1, ETF1, ERF1_HUMAN, eRF1, ERF, D5S1995, Cl1 protein
交叉反应
Human
验证活性
1. Western blot analysis of eRF1 on human eRF1 recombinant protein using anti-eRF1 antibody at 1/1,000 dilution.
2. Western blot analysis of eRF1 on HEK293 (1) and eRF1-hIgGFc transfected HEK293 (2) cell lysate using anti-eRF1 antibody at 1/1,000 dilution.
3. Western blot analysis of eRF1 on different cell lysate using anti-eRF1 antibody at 1/1,000 dilution. Positive control: Lane1: MCF-7, Lane2: T47D, Lane3: MOLT4, Lane4: Raji.
4. Flow cytometric analysis of Hela cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
5. Flow cytometric analysis of HepG2 cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
应用
WB
FCM
推荐剂量
WB: 1:500-2000; FCM: 1:100-200
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Translation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. This complex performs a dual role, where, in the "GTP state," it controls the positioning of eRF1 toward the stop codon and peptidyl-tRNA, and, in the "GDP state," it promotes the release of the eRFs from the ribosome. eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is essential for the interaction of eRF1 with the ribosome. The gene encoding human eRF1 maps to chromosome 5q31.2.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P62495
化学信息
分子量Theoretical: 49 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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