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Anti-Phospho-STAT3 (Ser727) Antibody (8J881)

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Anti-Phospho-STAT3 (Ser727) Antibody (8J881)
产品编号 TMAB-01507

Anti-Phospho-STAT3 (Ser727) Antibody (8J881) 是一种 Rabbit 抗体，靶向 Phospho-STAT3 (Ser727)。Anti-Phospho-STAT3 (Ser727) Antibody (8J881) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

Anti-Phospho-STAT3 (Ser727) Antibody (8J881)

Anti-Phospho-STAT3 (Ser727) Antibody (8J881)

Rating icon 还可以
Anti-Phospho-STAT3 (Ser727) Antibody (8J881)
产品编号 TMAB-01507

Anti-Phospho-STAT3 (Ser727) Antibody (8J881) 是一种 Rabbit 抗体，靶向 Phospho-STAT3 (Ser727)。Anti-Phospho-STAT3 (Ser727) Antibody (8J881) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

规格价格库存数量
25 μL
¥ 790
5日内发货
50 μL
¥ 1,395
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-STAT3 (Ser727) Antibody (8J881) is a Rabbit antibody targeting Phospho-STAT3 (Ser727). Anti-Phospho-STAT3 (Ser727) Antibody (8J881) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
克隆号
8J881
交叉反应
Human,Rat,Mouse
验证活性
1. Sample:
Heart (Rat) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-phospho-STAT3 (Ser727) (TMAB-01507) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 94 kDa
2. Blank control: A431. Primary Antibody (green line): Rabbit Anti-phospho-STAT3 (Ser727) antibody (TMAB-01507)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: A549 (Human) Cell Lysate at 30 μg
Primary:
Anti-phospho-STAT3 (Ser727) (TMAB-01507) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92/84 kDa
Observed band size: 92/84 kDa
4. A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-STAT3 (Ser727)) monoclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
18. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
19. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (TMAB-01507) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
应用
WB
IHC-P
IHC-Fr
ICC/IF
IF
FCM
推荐剂量
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:20-50
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
抗原信息
免疫原
A synthesized peptide: human STAT3 around the phosphorylation site of S727
抗原种属
Human
基因ID
6774
Uniprot ID
P40763
研究领域
STAT family,Transcription factors/regulators,Transcription factors,Surface molecules,STATs,Other factors,SARS Coronavirus,STATs,Intracellular
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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